ArticleThe Biochemical journal2001
Identification and characterization of DdPDE3, a cGMP-selective phosphodiesterase from Dictyostelium.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Diverse Roles of the Multiple Phosphodiesterases in the Regulation of Cyclic Nucleotide Signaling inCells · 2025Review
- Analysis of cGMP Signaling in Dictyostelium.Methods in molecular biology (Clifton, N.J.) · 2024Article
- In Search of Enzymes with a Role in 3', 5'-Cyclic Guanosine Monophosphate Metabolism in Plants.Frontiers in plant science · 2016Review
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
- Disruption of a Plasmodium falciparum cyclic nucleotide phosphodiesterase gene causes aberrant gametogenesis.Molecular microbiology · 2008Article
- Gametogenesis in malaria parasites is mediated by the cGMP-dependent protein kinase.PLoS biology · 2008Article
- GBF-dependent family genes morphologically suppress the partially active Dictyostelium STATa strain.Development genes and evolution · 2008Article
- Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP.The Biochemical journal · 2007Article
- Cyclic GMP-specific phosphodiesterase-5 regulates motility of sea urchin spermatozoa.Molecular biology of the cell · 2006Article
- Identification of a novel type of cGMP phosphodiesterase that is defective in the chemotactic stmF mutants.Molecular biology of the cell · 2002Article
- Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in dictyostelium.Molecular biology of the cell · 2002Article
- A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium.The EMBO journal · 2002Article
- A model for cGMP signal transduction in Dictyostelium in perspective of 25 years of cGMP research.Journal of muscle research and cell motility · 2002Review
- cAMP signaling in Dictyostelium. Complexity of cAMP synthesis, degradation and detection.Journal of muscle research and cell motility · 2002Review
- Signaling pathways regulating Dictyostelium myosin II.Journal of muscle research and cell motility · 2002Review
- A contextual framework for characterizing motility and chemotaxis mutants in Dictyostelium discoideum.Journal of muscle research and cell motility · 2002Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Dictyostelium cAMP and cGMP have important functions as first and second messengers in chemotaxis and development. Two cyclic-nucleotide phosphodiesterases (DdPDE 1 and 2) have been identified previously, an extracellular dual-specificity enzyme and an intracellular cAMP-specific enzyme (encoded by the psdA and regA genes respectively). Biochemical data suggest the presence of at least one cGMP-specific phosphodiesterase (PDE) that is activated by cGMP. Using bioinformatics we identified a partial sequence in the Dictyostelium expressed sequence tag database that shows a high degree of amino acid sequence identity with mammalian PDE catalytic domains (DdPDE3). The deduced amino acid sequence of a full-length DdPDE3 cDNA isolated in this study predicts a 60 kDa protein with a 300-residue C-terminal PDE catalytic domain, which is preceded by approx. 200 residues rich in asparagine and glutamine residues. Expression of the DdPDE3 catalytic domain in Escherichia coli shows that the enzyme has Michaelis-Menten kinetics and a higher affinity for cGMP (K(m)=0.22 microM) than for cAMP (K(m)=145 microM); cGMP does not stimulate enzyme activity. The enzyme requires bivalent cations for activity; Mn(2+) is preferred to Mg(2+), whereas Ca(2+) yields no activity. DdPDE3 is inhibited by 3-isobutyl-1-methylxanthine with an IC(50) of approx. 60 microM. Overexpression of the DdPDE3 catalytic domain in Dictyostelium confirms these kinetic properties without indications of its activation by cGMP. The properties of DdPDE3 resemble those of mammalian PDE9, which also shows the highest sequence similarity within the catalytic domains. DdPDE3 is the first cGMP-selective PDE identified in lower eukaryotes.
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