ArticleJournal of medical virology2001
Reverse transcription polymerase chain reaction (RT-PCR) for diagnosis of respiratory syncytial virus infection in adults: use of a single-tube "hanging droplet" nested PCR.
Article in Journal of medical virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 48 citations in OpenAlex.
- COVID-19 Classification from Chest X-Ray Images: A Framework of Deep Explainable Artificial Intelligence.Computational intelligence and neuroscience · 2022Article
- A State-of-the-Art Survey on Artificial Intelligence to Fight COVID-19.Journal of clinical medicine · 2021Review
- A highly sensitive 1-tube nested real-time RT-PCR assay using LNA-modified primers for detection of respiratory syncytial virus.Diagnostic microbiology and infectious disease · 2019Article
- Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a single-tube 'hanging droplet' PCR assay with modified pan-PV CODEHOP primers.The Journal of general virology · 2018Article
- Influenza-like illness sentinel surveillance in one hospital in Medellin, Colombia. 2007-2012.Influenza and other respiratory viruses · 2015Article
- Circulating human antibody-secreting cells during vaccinations and respiratory viral infections are characterized by high specificity and lack of bystander effect.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Article
- Detection of respiratory syncytial virus in adults with chronic obstructive pulmonary disease.American journal of respiratory and critical care medicine · 2006Article
- Respiratory viral infections in patients with chronic, obstructive pulmonary disease.The Journal of infection · 2005Article
- Comparison of quantitative reverse transcription-PCR to viral culture for assessment of respiratory syncytial virus shedding.Journal of clinical microbiology · 2003Article
- GeneScan reverse transcription-PCR assay for detection of six common respiratory viruses in young children hospitalized with acute respiratory illness.Journal of clinical microbiology · 2003Article
- Applicability of a real-time quantitative PCR assay for diagnosis of respiratory syncytial virus infection in immunocompromised adults.Journal of clinical microbiology · 2003Article
- Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2003Article
- Sensitive detection of RNA viruses associated with gastroenteritis by a hanging-drop single-tube nested reverse transcription-PCR method.Journal of clinical microbiology · 2002Article
- Diagnosis of respiratory syncytial virus infection: comparison of reverse transcription-PCR to viral culture and serology in adults with respiratory illness.Journal of clinical microbiology · 2002Article
- [Application of molecular assay for adenovirus detection among different pediatric patients].Revista paulista de pediatria : orgao oficial da Sociedade de Pediatria de Sao PauloArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Rapid diagnosis of Respiratory Syncytial virus (RSV) infection is difficult in elderly persons due to the low quantities of virus shed. Therefore, reverse transcription-polymerase chain reaction (RT-PCR) was used to detect viral RNA in respiratory secretions. A single-tube nested RT-PCR that used primers from a conserved F gene sequence was developed using a "hanging droplet" to physically separate outer and inner primer pairs during the first round of the PCR reaction. This was accomplished by placing the inner primers in a 5 microL droplet on the underside on the reaction tube cap and mixing after the first round of PCR. As few as 0.05 pfu of virus could be detected and gave positive results with RSV strains that represented the major groups and subgroups of RSV grown in tissue culture. The nested PCR was approximately 100-fold more sensitive than standard single primer PCR reactions and equivalent to standard two-tube nested PCR. Viral RNA was detected in nasopharyngeal samples from 12 of 15 culture positive illnesses and in 5 of 17 culture-negative, seropositive illnesses despite specimen volumes less than 1 microL in some samples. The method was also positive in 14 of 25 elderly volunteers inoculated with a live attenuated RSV vaccine candidate, only one of whom was culture positive. Use of a nested RT-PCR significantly improves the ability to detect RSV in respiratory samples and should improve the ability to rapidly diagnose RSV infection in adults, especially in the elderly.
Indexed as
Identifiers
11170067PMC7166324W2067557457What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.