ReviewCurrent opinion in biotechnology2001
Enzyme fluorescence as a sensing tool: new perspectives in biotechnology.
Review in Current opinion in biotechnology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed.
- Sequence-Defined Short Peptide-Derived Coacervate Vesicles for Targeted Therapeutics.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Theranostics in the management of colorectal cancer.Discover nano · 2025Review
- Bio-fabrication of thermozyme-based nano-biosensors: their components and present scenario.Journal of materials science. Materials in electronics · 2022Article
- A Fluorescent Biosensors for Detection Vital Body Fluids' Agents.Sensors (Basel, Switzerland) · 2018Review
- Sensing of Salivary Glucose Using Nano-Structured Biosensors.Biosensors · 2016Article
- Enzyme reactions in nanoporous, picoliter volume containers.Analytical chemistry · 2012Article
- Simultaneous determination of glucose and choline based on the intrinsic fluorescence of the enzymes.Journal of fluorescence · 2009Article
- Fluorescence patterns from supramolecular polymer assembly and disassembly for sensing metallo- and nonmetalloproteins.Journal of the American Chemical Society · 2009Article
- Glucose Determination by Means of Steady-state and Time-course UV Fluorescence in Free or Immobilized Glucose Oxidase.Sensors (Basel, Switzerland) · 2007Article
- Structural and thermal stability characterization of Escherichia coli D-galactose/D-glucose-binding protein.Biotechnology progressArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The technology for fluorescence protein-sensing is advancing rapidly owing to the continued introduction of new concepts, new fluorophores, and proteins engineered for sensing-specific analytes. Concerns about the reversibility and selectivity of engineered proteins are being addressed by developing biosensors that are based on the utilisation of coenzyme-depleted enzymes. Such biomolecules do not consume the substrate and can exhibit conformational changes upon the binding of the analyte, which can be easily detected as fluorescence change. In addition, concerns about the stability of biosensors can be overcome by using thermostable enzymes isolated from thermophilic microorganisms. Finally, the development of new techniques such as polarization-based sensing, anisotropy-based sensing and lifetime-based sensing, all of which can be accomplished with light-emitting diodes as the light source, is prompting the design of a new class of specific and stable biosensors, as has occurred with blood glucose measurement. These biosensors represent a valid alternative to the conventional clinical chemistry diagnostics.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.