ArticlePlant physiology2001
Specific binding of vf14-3-3a isoform to the plasma membrane H+-ATPase in response to blue light and fusicoccin in guard cells of broad bean.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 89 citations in OpenAlex.
- Plant Stomata: An Unrealized Possibility in Plant Defense against Invading Pathogens and Stress Tolerance.Plants (Basel, Switzerland) · 2023Review
- Identification of Abscisic Acid-Dependent Phosphorylated Basic Helix-Loop-Helix Transcription Factors in Guard Cells ofFrontiers in plant science · 2021Article
- Guard Cells Integrate Light and Temperature Signals to Control Stomatal Aperture.Plant physiology · 2020Article
- The Role of the Plasma Membrane HFrontiers in plant science · 2017Review
- 14-3-3 Proteins in Guard Cell Signaling.Frontiers in plant science · 2015Review
- Long-chain bases, phosphatidic acid, MAPKs, and reactive oxygen species as nodal signal transducers in stress responses in Arabidopsis.Frontiers in plant science · 2015Article
- Guard cell chloroplasts are essential for blue light-dependent stomatal opening in Arabidopsis.PloS one · 2014Article
- CYCLIN H;1 regulates drought stress responses and blue light-induced stomatal opening by inhibiting reactive oxygen species accumulation in Arabidopsis.Plant physiology · 2013Article
- Identification of lipids and lipid-binding proteins in phloem exudates from Arabidopsis thaliana.Journal of experimental botany · 2012Article
- New aspects of Phloem-mediated long-distance lipid signaling in plants.Frontiers in plant science · 2012Article
- 14-3-3 phosphoprotein interaction networks - does isoform diversity present functional interaction specification?Frontiers in plant science · 2012Article
- Control of stomatal aperture: a renaissance of the old guard.Plant signaling & behavior · 2011Review
- The cytosolic/nuclear HSC70 and HSP90 molecular chaperones are important for stomatal closure and modulate abscisic acid-dependent physiological responses in Arabidopsis.Plant physiology · 2011Article
- Molecular battles between plant and pathogenic bacteria in the phyllosphere.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2010Article
- Phosphatidic acid inhibits blue light-induced stomatal opening via inhibition of protein phosphatase 1 [corrected].Plant physiology · 2010Article
- Stomata and pathogens: Warfare at the gates.Plant signaling & behavior · 2009Review
- Xanthomonas campestris overcomes Arabidopsis stomatal innate immunity through a DSF cell-to-cell signal-regulated virulence factor.Plant physiology · 2009Article
- Phospholipase A2beta mediates light-induced stomatal opening in Arabidopsis.Journal of experimental botany · 2008Article
- Constitutive activation of a plasma membrane H(+)-ATPase prevents abscisic acid-mediated stomatal closure.The EMBO journal · 2007Article
- Isolation of a protein interacting with Vfphot1a in guard cells of Vicia faba.Plant physiology · 2005Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plasma membrane H(+)-ATPase is activated by blue light with concomitant binding of the 14-3-3 protein to the C terminus in guard cells. Because several isoforms of the 14-3-3 protein are expressed in plants, we determined which isoform(s) bound to the H(+)-ATPase in vivo. Four cDNA clones (vf14-3-3a, vf14-3-3b, vf14-3-3c, and vf14-3-3d) encoding 14-3-3 proteins were isolated from broad bean (Vicia faba) guard cells. Northern analysis revealed that mRNAs encoding vf14-3-3a and vf14-3-3b proteins were expressed predominantly in guard cells. The 14-3-3 protein that bound to the H(+)-ATPase in guard cells had the same molecular mass as the recombinant vf14-3-3a protein. The H(+)-ATPase immunoprecipitated from mesophyll cell protoplasts, which had been stimulated by fusicoccin, coprecipitated with the 32.5-kD 14-3-3 protein, although three 14-3-3 isoproteins were found in mesophyll cell protoplasts. Digestions of the bound 14-3-3 protein and recombinant vf14-3-3a with cyanogen bromide gave the identical migration profiles on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, but that of vf14-3-3b gave a different profile. Mass profiling of trypsin-digested 14-3-3 protein bound to the H(+)-ATPase gave the predicted peptide masses of vf14-3-3a. Far western analysis revealed that the H(+)-ATPase had a higher affinity for vf14-3-3a than for vf14-3-3b. These results suggest that the 14-3-3 protein that bound to the plasma membrane H(+)-ATPase in vivo is vf14-3-3a and that it may play a key role in the activation of H(+)-ATPase in guard cells.
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