ArticlePlant physiology2001
Overexpression of 3-ketoacyl-acyl-carrier protein synthase IIIs in plants reduces the rate of lipid synthesis.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed, 169 citations in OpenAlex.
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- Genetic and Morpho-Physiological Differences among Transgenic and No-Transgenic Cotton Cultivars.Plants (Basel, Switzerland) · 2023Article
- The PRK/Rubisco shunt strongly influences Arabidopsis seed metabolism and oil accumulation, affecting more than carbon recycling.The Plant cell · 2023Article
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- Domestication and improvement genes reveal the differences of seed size- and oil-related traits in soybean domestication and improvement.Computational and structural biotechnology journal · 2022Article
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- A Novel Bifunctional Wax Ester Synthase Involved in Early Triacylglycerol Accumulation in Unicellular Green MicroalgaFrontiers in bioengineering and biotechnology · 2021Article
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- Quantitative trait loci analysis of seed oil content and composition of wild and cultivated soybean.BMC plant biology · 2020Article
- Short Chain Fatty Acid Biosynthesis in MicroalgaeMarine drugs · 2019Article
- Understanding the functions of endogenous DOF transcript factor inBiotechnology for biofuels · 2019Article
- Comparative Transcriptome Analysis Reveals an Efficient Mechanism of α-Linolenic Acid in Tree Peony Seeds.International journal of molecular sciences · 2018Article
- Analysis of Interdependent Kinetic Controls of Fatty Acid Synthases.ACS catalysis · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA coding for 3-ketoacyl-acyl-carrier protein (ACP) synthase III (KAS III) from spinach (Spinacia oleracea; So KAS III) was used to isolate two closely related KAS III clones (Ch KAS III-1 and Ch KAS III-2) from Cuphea hookeriana. Both Ch KAS IIIs are expressed constitutively in all tissues examined. An increase in the levels of 16:0 was observed in tobacco (Nicotiana tabacum, WT-SR) leaves overexpressing So KAS III when under the control of the cauliflower mosaic virus-35S promoter and in Arabidopsis and rapeseed (Brassica napus) seeds overexpressing either of the Ch KAS IIIs driven by napin. These data indicate that this enzyme has a universal role in fatty acid biosynthesis, irrespective of the plant species from which it is derived or the tissue in which it is expressed. The transgenic rapeseed seeds also contained lower levels of oil as compared with the wild-type levels. In addition, the rate of lipid synthesis in transgenic rapeseed seeds was notably slower than that of the wild-type seeds. The results of the measurements of the levels of the acyl-ACP intermediates as well as any changes in levels of other fatty acid synthase enzymes suggest that malonyl-ACP, the carbon donor utilized by all the 3- ketoacyl-ACP synthases, is limiting in the transgenic plants. This further suggests that malonyl-coenzyme A is a potential limiting factor impacting the final oil content as well as further extension of 16:0.
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