ArticlePlant physiology2001
Redox control of ntcA gene expression in Synechocystis sp. PCC 6803. Nitrogen availability and electron transport regulate the levels of the NtcA protein.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 51 citations in OpenAlex.
- Green Extraction and Fractionation of Chestnut Wood Waste: A Sustainable Pathway to Biopolymers and Antimicrobial Solutions.ChemSusChem · 2025Article
- A mechanistic study of the influence of nitrogen and energy availability on the NH4+ sensitivity of nitrogen assimilation in Synechococcus.Journal of experimental botany · 2022Article
- MarineMicrobiology spectrum · 2022Article
- A Novel Mechanism, Linked to Cell Density, Largely Controls Cell Division inPlant physiology · 2017Article
- Cyanobacterial nitrogenases: phylogenetic diversity, regulation and functional predictions.Genetics and molecular biology · 2017Article
- Physiological Studies of Glutamine Synthetases I and III from Synechococcus sp. WH7803 Reveal Differential Regulation.Frontiers in microbiology · 2016Article
- Nitrogen Starvation Acclimation in Synechococcus elongatus: Redox-Control and the Role of Nitrate Reduction as an Electron Sink.Life (Basel, Switzerland) · 2015Article
- An integrative approach for modeling and simulation of heterocyst pattern formation in cyanobacteria filaments.PLoS computational biology · 2015Article
- Wide-dynamic-range promoters engineered for cyanobacteria.Journal of biological engineering · 2013Article
- The languages spoken in the water body (or the biological role of cyanobacterial toxins).Frontiers in microbiology · 2012Article
- Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology.Nucleic acids research · 2010Article
- Expression of genes involved in nitrogen assimilation and the C/N balance sensing in Prochlorococcus sp. strain SS120.Gene expression · 2009Article
- Gene expression patterns of sulfur starvation in Synechocystis sp. PCC 6803.BMC genomics · 2008Article
- Coordinated high-light response of genes encoding subunits of photosystem I is achieved by AT-rich upstream sequences in the cyanobacterium Synechocystis sp. strain PCC 6803.Journal of bacteriology · 2007Article
- A pair of iron-responsive genes encoding protein kinases with a Ser/Thr kinase domain and a His kinase domain are regulated by NtcA in the Cyanobacterium Anabaena sp. strain PCC 7120.Journal of bacteriology · 2006Article
- A LexA-related protein regulates redox-sensitive expression of the cyanobacterial RNA helicase, crhR.Nucleic acids research · 2006Article
- Comparative genomics analysis of NtcA regulons in cyanobacteria: regulation of nitrogen assimilation and its coupling to photosynthesis.Nucleic acids research · 2005Article
- HetR homodimer is a DNA-binding protein required for heterocyst differentiation, and the DNA-binding activity is inhibited by PatS.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Nitrate/nitrite assimilation system of the marine picoplanktonic cyanobacterium Synechococcus sp. strain WH 8103: effect of nitrogen source and availability on gene expression.Applied and environmental microbiology · 2003Article
- DNA microarray analysis of redox-responsive genes in the genome of the cyanobacterium Synechocystis sp. strain PCC 6803.Journal of bacteriology · 2003Article
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3 authors at 2 institutions in 1 country.
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Abstract
In this work we have studied the influence of the cellular redox status in the expression of the Synechocystis sp. PCC 6803 ntcA gene. Two different ntcA transcripts with different 5' ends were detected, depending on the different dark/light or nitrogen availability conditions. Accumulation of a 0.8-kb ntcA message was light and nitrogen dependent, whereas a longer 1.2-kb ntcA transcript was neither light nor nitrogen regulated. NtcA protein levels increased concomitantly with the accumulation of the 0.8-kb ntcA transcript. The light-dependent accumulation of the ntcA gene and the NtcA protein was sensitive to electron transport inhibitors. In addition, Glc-grown Synechocystis sp. cells showed a similar ntcA expression pattern in darkness to that observed under illumination. These data suggested that electron transport, and not light per se may regulate ntcA gene expression. Primer extension analysis, together with gel mobility-shift assays, demonstrated that in vitro, the Synechocystis sp. NtcA protein specifically bound to the putative promoter region from the light/nitrogen-dependent ntcA transcript but not to that from the constitutive 1.2-kb ntcA mRNA. Band-shift experiments carried out in the presence of thiol oxidizing/modifiying agents and different reducing/oxidizing conditions suggested that NtcA binding to its own promoter was under a thiol-dependent redox mechanism. Our results suggest that the cellular redox status plays a central role in the autoregulatory mechanism of the NtcA protein.
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