ArticleNucleic acids research2001
A novel multiple affinity purification tag and its use in identification of proteins associated with a cyclin-CDK complex.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Identification of a Common Epitope in Nucleocapsid Proteins of Euro-America Orthotospoviruses and Its Application for Tagging Proteins.International journal of molecular sciences · 2021Article
- Interaction sites of the Epstein-Barr virus Zta transcription factor with the host genome in epithelial cells.Access microbiology · 2021Article
- Purification of Cyclin-Dependent Kinase Fusion Complexes for In Vitro Analysis.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Comparison of CRISPR Genomic Tagging for Affinity Purification and Endogenous Immunoprecipitation Coupled with Quantitative Mass Spectrometry To Identify the Dynamic AMPKα2 Interactome.Journal of proteome research · 2019Article
- HiBiT-qIP, HiBiT-based quantitative immunoprecipitation, facilitates the determination of antibody affinity under immunoprecipitation conditions.Scientific reports · 2019Article
- Comparison of the activity of three different HSP70 inhibitors on apoptosis, cell cycle arrest, autophagy inhibition, and HSP90 inhibition.Cancer biology & therapy · 2014Article
- Redundancy or specificity? The role of the CDK Pho85 in cell cycle control.International journal of biochemistry and molecular biology · 2013Review
- Several affinity tags commonly used in chromatographic purification.Journal of analytical methods in chemistry · 2013Review
- Highly efficient purification of protein complexes from mammalian cells using a novel streptavidin-binding peptide and hexahistidine tandem tag system: application to Bruton's tyrosine kinase.Protein science : a publication of the Protein Society · 2011Article
- Entamoeba histolytica sirtuin EhSir2a deacetylates tubulin and regulates the number of microtubular assemblies during the cell cycle.Cellular microbiology · 2010Article
- A rapid and universal tandem-purification strategy for recombinant proteins.Protein science : a publication of the Protein Society · 2007Article
- Bayesian Orthogonal Least Squares (BOLS) algorithm for reverse engineering of gene regulatory networks.BMC bioinformatics · 2007Article
- Roles of the CDK phosphorylation sites of yeast Cdc6 in chromatin binding and rereplication.Molecular biology of the cell · 2007Article
- Recruitment of Cdc28 by Whi3 restricts nuclear accumulation of the G1 cyclin-Cdk complex to late G1.The EMBO journal · 2004Article
- Physical methods.Plant molecular biology · 2002Article
- Strains of [PSI(+)] are distinguished by their efficiencies of prion-mediated conformational conversion.The EMBO journal · 2001Article
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Authors and funding
5 authors.
Funding
Abstract
A novel multiple affinity purification (MAFT) or tandem affinity purification (TAP) tag has been constructed. It consists of the calmodulin binding peptide, six histidine residues, and three copies of the hemagglutinin epitope. This 'CHH' MAFT tag allows two or three consecutive purification steps, giving high purity. Active Clb2-Cdc28 kinase complex was purified from yeast cells after inserting the CHH tag into Clb2. Associated proteins were identified using mass spectrometry. These included the known associated proteins Cdc28, Sic1 and Cks1. Several other proteins were found including the 70 kDa chaperone, Ssa1.
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