ArticleNucleic acids research2001
A novel approach for the identification of protein-protein interaction with integral membrane proteins.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Unravelling the Phosphorylation Landscape of Anthrax Toxin Receptor 1: Mechanisms and Functional Insights Through Phosphoproteomic Data Integration.The protein journal · 2025Article
- Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Getting to know each other: PPIMem, a novel approach for predicting transmembrane protein-protein complexes.Computational and structural biotechnology journal · 2021Article
- Review
- Predictive and Experimental Approaches for Elucidating Protein-Protein Interactions and Quaternary Structures.International journal of molecular sciences · 2017Review
- A novel genetic system based on zinc finger nucleases for the identification of interactions between proteins in vivo.PloS one · 2013Article
- Ceramide kinase-like (CERKL) interacts with neuronal calcium sensor proteins in the retina in a cation-dependent manner.Investigative ophthalmology & visual science · 2012Article
- Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiology and molecular biology reviews : MMBR · 2012Review
- Functional characterization of SAMD9, a protein deficient in normophosphatemic familial tumoral calcinosis.The Journal of investigative dermatology · 2011Article
- The Sos-recruitment system as a tool to analyze cellular localization of plant proteins: membrane localization of Arabidopsis thaliana PEPINO/PASTICCINO2.Molecular genetics and genomics : MGG · 2010Article
- A novel c-Jun N-terminal kinase (JNK)-binding protein WDR62 is recruited to stress granules and mediates a nonclassical JNK activation.Molecular biology of the cell · 2010Article
- Yeast two-hybrid, a powerful tool for systems biology.International journal of molecular sciences · 2009Review
- Examining protein protein interactions using endogenously tagged yeast arrays: the cross-and-capture system.Genome research · 2007Article
- RalA interacts with ZONAB in a cell density-dependent manner and regulates its transcriptional activity.The EMBO journal · 2005Article
- The yeast split-ubiquitin membrane protein two-hybrid screen identifies BAP31 as a regulator of the turnover of endoplasmic reticulum-associated protein tyrosine phosphatase-like B.Molecular and cellular biology · 2004Article
- Identification of novel ErbB3-interacting factors using the split-ubiquitin membrane yeast two-hybrid system.Genome research · 2003Article
- Adaptation of the Ras-recruitment system to the analysis of interactions between membrane-associated proteins.Nucleic acids research · 2003Article
- Identification and characterization of a tissue-specific coactivator, GT198, that interacts with the DNA-binding domains of nuclear receptors.Molecular and cellular biology · 2002Article
- The bZIP repressor proteins, c-Jun dimerization protein 2 and activating transcription factor 3, recruit multiple HDAC members to the ATF3 promoter.Biochimica et biophysica actaArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein-protein interaction plays a major role in all biological processes. The currently available genetic methods such as the two-hybrid system and the protein recruitment system are relatively limited in their ability to identify interactions with integral membrane proteins. Here we describe the development of a reverse Ras recruitment system (reverse RRS), in which the bait used encodes a membrane protein. The bait is expressed in its natural environment, the membrane, whereas the protein partner (the prey) is fused to a cytoplasmic Ras mutant. Protein-protein interaction between the proteins encoded by the prey and the bait results in Ras membrane translocation and activation of a viability pathway in yeast. We devised the expression of the bait and prey proteins under the control of dual distinct inducible promoters, thus enabling a rapid selection of transformants in which growth is attributed solely to specific protein-protein interaction. The reverse RRS approach greatly extends the usefulness of the protein recruitment systems and the use of integral membrane proteins as baits. The system serves as an attractive approach to explore novel protein-protein interactions with high specificity and selectivity, where other methods fail.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.