ArticleNucleic acids research2001
Rapid evolution of the DNA-binding site in LAGLIDADG homing endonucleases.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 75 citations in OpenAlex.
- RHO1-2 meganuclease gene editing targets human P23H rhodopsin-induced retinitis pigmentosa to rejuvenate rods and maintain cones.bioRxiv : the preprint server for biology · 2025Article
- Accurate, comprehensive database of group I introns and their homing endonucleases.Bioinformatics advances · 2025Article
- Description ofPhytoKeys · 2024Article
- Mitochondrial and Plastid Genomes of the Monoraphid DiatomInternational journal of molecular sciences · 2021Article
- The Coevolution of Fungal Mitochondrial Introns and Their Homing Endonucleases (GIY-YIG and LAGLIDADG).Genome biology and evolution · 2020Article
- Modifying a covarying protein-DNA interaction changes substrate preference of a site-specific endonuclease.Nucleic acids research · 2019Article
- Article
- Crystal Structure of the Homing Endonuclease I-CvuI Provides a New Template for Genome Modification.The Journal of biological chemistry · 2015Article
- Article
- Crystallization and preliminary X-ray diffraction analysis of the homing endonuclease I-CvuI from Chlorella vulgaris in complex with its target DNA.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Article
- 5'-Cytosine-phosphoguanine (CpG) methylation impacts the activity of natural and engineered meganucleases.The Journal of biological chemistry · 2012Article
- Expanding LAGLIDADG endonuclease scaffold diversity by rapidly surveying evolutionary sequence space.Nucleic acids research · 2012Article
- Comprehensive homing endonuclease target site specificity profiling reveals evolutionary constraints and enables genome engineering applications.Nucleic acids research · 2012Article
- Native homing endonucleases can target conserved genes in humans and in animal models.Nucleic acids research · 2011Article
- Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.Current gene therapy · 2011Review
- Evolution of I-SceI homing endonucleases with increased DNA recognition site specificity.Journal of molecular biology · 2011Article
- A group II intron encodes a functional LAGLIDADG homing endonuclease and self-splices under moderate temperature and ionic conditions.RNA (New York, N.Y.) · 2010Article
- Group I introns and inteins: disparate origins but convergent parasitic strategies.Journal of bacteriology · 2009Review
- Post-transcriptional control of chloroplast gene expression.Gene regulation and systems biology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequence analysis of chloroplast and mitochondrial large subunit rRNA genes from over 75 green algae disclosed 28 new group I intron-encoded proteins carrying a single LAGLIDADG motif. These putative homing endonucleases form four subfamilies of homologous enzymes, with the members of each subfamily being encoded by introns sharing the same insertion site. We showed that four divergent endonucleases from the I-CreI subfamily cleave the same DNA substrates. Mapping of the 66 amino acids that are conserved among the members of this subfamily on the 3-dimensional structure of I-CreI bound to its recognition sequence revealed that these residues participate in protein folding, homodimerization, DNA recognition and catalysis. Surprisingly, only seven of the 21 I-CreI amino acids interacting with DNA are conserved, suggesting that I-CreI and its homologs use different subsets of residues to recognize the same DNA sequence. Our sequence comparison of all 45 single-LAGLIDADG proteins identified so far suggests that these proteins share related structures and that there is a weak pressure in each subfamily to maintain identical protein-DNA contacts. The high sequence variability we observed in the DNA-binding site of homologous LAGLIDADG endonucleases provides insight into how these proteins evolve new DNA specificity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.