ArticleNucleic acids research2001
Identification and properties of the crenarchaeal single-stranded DNA binding protein from Sulfolobus solfataricus.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
68 citing papers in PubMed, 134 citations in OpenAlex.
- Response Factor Correction for Quantitative Determination of HomooligomericJournal of the American Society for Mass Spectrometry · 2026Article
- Capture of RNA-binding proteins across mouse tissues using HARD-AP.Nature communications · 2024Article
- Identification and Preliminary Characterization of a Novel Single-Stranded DNA Binding Protein of Staphylococcus aureus Phage Phi11 Expressed in Escherichia coli.Molecular biotechnology · 2023Article
- DNA-binding mechanism and evolution of replication protein A.Nature communications · 2023Article
- A Novel Family of Winged-Helix Single-Stranded DNA-Binding Proteins from Archaea.International journal of molecular sciences · 2022Article
- The Finely Coordinated Action of SSB and NurA/HerA Complex Strictly Regulates the DNA End Resection Process inInternational journal of molecular sciences · 2022Article
- Take a Break to Repair: A Dip in the World of Double-Strand Break Repair Mechanisms Pointing the Gaze on Archaea.International journal of molecular sciences · 2021Review
- Single-Stranded DNA-Binding Proteins in the Archaea.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Unlimited Cooperativity ofFrontiers in microbiology · 2021Article
- The Essential, Ubiquitous Single-Stranded DNA-Binding Proteins.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Review
- A Structural Perspective on the Regulation of Human Single-Stranded DNA Binding Protein 1 (hSSB1, OBFC2B) Function in DNA Repair.Computational and structural biotechnology journal · 2019Review
- Snapshots of archaeal DNA replication and repair in living cells using super-resolution imaging.Nucleic acids research · 2018Article
- Prespacer processing and specific integration in a Type I-A CRISPR system.Nucleic acids research · 2018Article
- Primer synthesis by a eukaryotic-like archaeal primase is independent of its Fe-S cluster.Nature communications · 2017Article
- A data-driven structural model of hSSB1 (NABP2/OBFC2B) self-oligomerization.Nucleic acids research · 2017Article
- High-affinity RNA binding by a hyperthermophilic single-stranded DNA-binding protein.Extremophiles : life under extreme conditions · 2017Article
- Staphylococcus aureus single-stranded DNA-binding protein SsbA can bind but cannot stimulate PriA helicase.PloS one · 2017Article
- A structural analysis of DNA binding by hSSB1 (NABP2/OBFC2B) in solution.Nucleic acids research · 2016Article
- A targeted DNA substrate mechanism for the inhibition of HIV-1 integrase by inhibitors with antiretroviral activity.FEBS open bio · 2016Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-stranded DNA binding proteins (SSBs) play central roles in cellular and viral processes involving the generation of single-stranded DNA. These include DNA replication, homologous recombination and DNA repair pathways. SSBs bind DNA using four 'OB-fold' (oligonucleotide/oligosaccharide binding fold) domains that can be organised in a variety of overall quaternary structures. Thus eubacterial SSBs are homotetrameric whilst the eucaryal RPA protein is a heterotrimer and euryarchaeal proteins vary significantly in their subunit compositions. We demonstrate that the crenarchaeal SSB protein is an abundant protein with a unique structural organisation, existing as a monomer in solution and multimerising on DNA binding. The protein binds single-stranded DNA distributively with a binding site size of approximately 5 nt per monomer. Sulfolobus SSB lacks the zinc finger motif found in the eucaryal and euryarchaeal proteins, possessing instead a flexible C-terminal tail, sensitive to trypsin digestion, that is not required for DNA binding. In comparison with Escherichia coli SSB, the tail may play a role in protein-protein interactions during DNA replication and repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.