ArticleNucleic acids research2001
A new method for generating point mutations in bacterial artificial chromosomes by homologous recombination in Escherichia coli.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 73 citations in OpenAlex.
- A combined pseudouridine biomanufacturing platform enabled by a streamlined designer pathway.Nature communications · 2025Article
- Versatile allelic replacement and self-excising integrative vectors for plasmid genome mutation and complementation.Microbiology spectrum · 2024Article
- Comparative genome mining and heterologous expression of an orphan NRPS gene cluster direct the production of ashimides.Chemical science · 2019Article
- Recent Advances in the Genetic Manipulation of Methylosinus trichosporium OB3b.Methods in enzymology · 2018Article
- Convergence of DNA methylation and phosphorothioation epigenetics in bacterial genomes.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The Autographa californica Multiple Nucleopolyhedrovirus ac54 Gene Is Crucial for Localization of the Major Capsid Protein VP39 at the Site of Nucleocapsid Assembly.Journal of virology · 2016Article
- Altered trafficking and stability of polycystins underlie polycystic kidney disease.The Journal of clinical investigation · 2014Article
- A single vector-based strategy for marker-less gene replacement in Synechocystis sp. PCC 6803.Microbial cell factories · 2014Article
- A bacterial artificial chromosome transgene with polymorphic Cd72 inhibits the development of glomerulonephritis and vasculitis in MRL-Faslpr lupus mice.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- The neuronal serum- and glucocorticoid-regulated kinase 1.1 reduces neuronal excitability and protects against seizures through upregulation of the M-current.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Activation of serum/glucocorticoid-induced kinase 1 (SGK1) is important to maintain skeletal muscle homeostasis and prevent atrophy.EMBO molecular medicine · 2013Article
- Alternative splicing and tissue-specific elastin misassembly act as biological modifiers of human elastin gene frameshift mutations associated with dominant cutis laxa.The Journal of biological chemistry · 2012Article
- Review
- Small-molecule control of protein degradation using split adaptors.ACS chemical biology · 2011Article
- A genetic interaction network of five genes for human polycystic kidney and liver diseases defines polycystin-1 as the central determinant of cyst formation.Nature genetics · 2011Article
- Critical role of the virus-encoded microRNA-155 ortholog in the induction of Marek's disease lymphomas.PLoS pathogens · 2011Article
- Human herpesvirus 6 major immediate early promoter has strong activity in T cells and is useful for heterologous gene expression.Virology journal · 2011Article
- Mutation of herpesvirus Saimiri ORF51 glycoprotein specifically targets infectivity to hepatocellular carcinoma cell lines.Journal of biomedicine & biotechnology · 2011Article
- BACs as tools for the study of genomic imprinting.Journal of biomedicine & biotechnology · 2011Review
- Functional analysis of Spodoptera litura nucleopolyhedrovirus p49 gene during Autographa californica nucleopolyhedrovirus infection of SpLi-221 cells.Virus genes · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Bacterial artificial chromosomes (BACs) and P1 artificial chromosomes (PACs), which contain large fragments of genomic DNA, have been successfully used as transgenes to create mouse models of dose-dependent diseases. They are also potentially valuable as transgenes for dominant diseases given that point mutations and/or small rearrangements can be accurately introduced. Here, we describe a new method to introduce small alterations in BACs, which results in the generation of point mutations with high frequency. The method involves homologous recombination between the original BAC and a shuttle vector providing the mutation. Each recombination step is monitored using positive and negative selection markers, which are the Kanamycin-resistance gene, the sacB gene and temperature-sensitive replication, all conferred by the shuttle plasmid. We have used this method to introduce four different point mutations and the insertion of the ss-galactosidase gene in a BAC, which has subsequently been used for transgenic animal production.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.