ArticleMolecular biology of the cell2001
Mutant Caldesmon lacking cdc2 phosphorylation sites delays M-phase entry and inhibits cytokinesis.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Glucocorticoid receptor-induced non-muscle caldesmon regulates metastasis in castration-resistant prostate cancer.Oncogenesis · 2023Article
- Revisiting the multisite phosphorylation that produces the M-phase supershift of key mitotic regulators.Molecular biology of the cell · 2022Article
- Article
- Cell adhesion is regulated by CDK1 during the cell cycle.The Journal of cell biology · 2018Article
- Article
- Caldesmon regulates axon extension through interaction with myosin II.The Journal of biological chemistry · 2012Article
- Caldesmon regulates actin dynamics to influence cranial neural crest migration in Xenopus.Molecular biology of the cell · 2011Article
- Phosphorylation of Caldesmon by PFTAIRE1 kinase promotes actin binding and formation of stress fibers.Molecular and cellular biochemistry · 2011Article
- Activation of cyclin B1-Cdk1 synchronizes events in the nucleus and the cytoplasm at mitosis.The Journal of cell biology · 2010Article
- Caldesmon regulates the motility of vascular smooth muscle cells by modulating the actin cytoskeleton stability.Journal of biomedical science · 2010Article
- Caldesmon as a therapeutic target for proliferative vascular diseases.Mini reviews in medicinal chemistry · 2008Review
- Caldesmon and the regulation of cytoskeletal functions.Advances in experimental medicine and biology · 2008Review
- ARL4D recruits cytohesin-2/ARNO to modulate actin remodeling.Molecular biology of the cell · 2007Article
- Phosphorylation of actopaxin regulates cell spreading and migration.The Journal of cell biology · 2004Article
- Postnatal changes in caldesmon expression and localization in cardiac myocytes.Journal of anatomy · 2003Article
- Alpha(v)beta3 integrin expression up-regulates cdc2, which modulates cell migration.The Journal of cell biology · 2003Article
- Article
- Actopaxin is phosphorylated during mitosis and is a substrate for cyclin B1/cdc2 kinase.The Biochemical journal · 2002Article
- Diversification of caldesmon-linked actin cytoskeleton in cell motility.Cell adhesion & migrationReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Caldesmon is phosphorylated by cdc2 kinase during mitosis, resulting in the dissociation of caldesmon from microfilaments. To understand the physiological significance of phosphorylation, we generated a caldesmon mutant replacing all seven cdc2 phosphorylation sites with Ala, and examined effects of expression of the caldesmon mutant on M-phase progression. We found that microinjection of mutant caldesmon effectively blocked early cell division of Xenopus embryos. Similar, though less effective, inhibition of cytokinesis was observed with Chinese hamster ovary (CHO) cells microinjected with 7th mutant. When mutant caldesmon was introduced into CHO cells either by protein microinjection or by inducible expression, delay of M-phase entry was observed. Finally, we found that 7th mutant inhibited the disassembly of microfilaments during mitosis. Wild-type caldesmon, on the other hand, was much less potent in producing these three effects. Because mutant caldesmon did not inhibit cyclin B/cdc2 kinase activity, our results suggest that alterations in microfilament assembly caused by caldesmon phosphorylation are important for M-phase progression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.