ArticleJournal of virology2001
Genetic and functional diversity of human immunodeficiency virus type 1 subtype B Nef primary isolates.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Natural HIV-1 Nef Polymorphisms Impair SERINC5 Downregulation Activity.Cell reports · 2019Article
- Article
- Nef is secreted in exosomes from Nef.GFP-expressing and HIV-1-infected human astrocytes.Journal of neurovirology · 2017Article
- Relative Resistance of HLA-B to Downregulation by Naturally Occurring HIV-1 Nef Sequences.mBio · 2016Article
- Primate lentiviral Nef proteins deregulate T-cell development by multiple mechanisms.Retrovirology · 2013Article
- HIV-1 Nef and T-cell activation: a history of contradictions.Future virology · 2013Article
- Overlapping effector interfaces define the multiple functions of the HIV-1 Nef polyproline helix.Retrovirology · 2012Article
- Mechanisms of HIV-1 Nef function and intracellular signaling.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2011Review
- Self-association of the Lentivirus protein, Nef.Retrovirology · 2010Article
- Meta-analysis to test the association of HIV-1 nef amino acid differences and deletions with disease progression.Journal of virology · 2010Article
- HIV-1 Nef: at the crossroads.Retrovirology · 2008Review
- Association of Nef with p21-activated kinase 2 is dispensable for efficient human immunodeficiency virus type 1 replication and cytopathicity in ex vivo-infected human lymphoid tissue.Journal of virology · 2007Article
- Article
- Primary human immunodeficiency virus type 1 nef alleles show major differences in pathogenicity in transgenic mice.Journal of virology · 2007Article
- Article
- Intra- and intersubtype alternative Pak2-activating structural motifs of human immunodeficiency virus type 1 Nef.Journal of virology · 2006Article
- A hydrophobic binding surface on the human immunodeficiency virus type 1 Nef core is critical for association with p21-activated kinase 2.Journal of virology · 2006Article
- Dynamic evolution of the human immunodeficiency virus type 1 pathogenic factor, Nef.Journal of virology · 2006Article
- Activation of p21-activated kinase 2 by human immunodeficiency virus type 1 Nef induces merlin phosphorylation.Journal of virology · 2005Article
- Reconstitution and molecular analysis of an active human immunodeficiency virus type 1 Nef/p21-activated kinase 2 complex.Journal of virology · 2005Article
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Authors and funding
7 authors.
Funding
Abstract
We have characterized the functional integrity of seven primary Nef isolates: five from a long-term nonprogressing human immunodeficiency virus (HIV)-infected individual and one each from two patients with AIDS. One of the seven Nefs was defective for CD4 downregulation, two others were defective for PAK-2 activation, and one Nef was defective for PAK-2 activation and major histocompatibility complex (MHC) class I downregulation. Five of the Nefs were tested and found to be functional for the enhancement of virus particle infectivity. The structural basis for each of the functional defects has been analyzed by constructing a consensus nef, followed by mutational analysis of the variant amino acid residues. Mutations A29V and F193I were deleterious to CD4 downregulation and PAK-2 activation, respectively, while S189R rendered Nef defective for both MHC class I downregulation and PAK-2 activation. A search of the literature identified HIVs from five patients with Nefs predominantly mutated at F193 and from one patient with Nefs predominantly mutated at A29. A29 is highly conserved in all HIV subtypes except for subtype E. F193 is conserved in subtype B (and possibly in the closely related subtype D), but none of the other HIV group M subtypes. Our results suggest that functional distinctions may exist between HIV subtypes.
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