ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2001
Interaction of stoned and synaptotagmin in synaptic vesicle endocytosis.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 97 citations in OpenAlex.
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- Flavonoid-Rich Ethanol Extract from the Leaves ofOxidative medicine and cellular longevity · 2018Article
- Safeguards of Neurotransmission: Endocytic Adaptors as Regulators of Synaptic Vesicle Composition and Function.Frontiers in cellular neuroscience · 2017Review
- Myosin light chain kinase facilitates endocytosis of synaptic vesicles at hippocampal boutons.Journal of neurochemistry · 2016Article
- Overlapping functions of stonin 2 and SV2 in sorting of the calcium sensor synaptotagmin 1 to synaptic vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- AP180 couples protein retrieval to clathrin-mediated endocytosis of synaptic vesicles.Traffic (Copenhagen, Denmark) · 2014Article
- Synaptic vesicle morphology: a case of protein sorting?Current opinion in cell biology · 2014Review
- Cargo recognition in clathrin-mediated endocytosis.Cold Spring Harbor perspectives in biology · 2013Review
- Drosophila-Cdh1 (Rap/Fzr) a regulatory subunit of APC/C is required for synaptic morphology, synaptic transmission and locomotion.International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience · 2013Article
- Adar is essential for optimal presynaptic function.Molecular and cellular neurosciences · 2013Article
- Cortical surface area correlates with STON2 gene Ser307Pro polymorphism in first-episode treatment-naïve patients with schizophrenia.PloS one · 2013Article
- Eps homology domain endosomal transport proteins differentially localize to the neuromuscular junction.Skeletal muscle · 2012Article
- UNC-41/stonin functions with AP2 to recycle synaptic vesicles in Caenorhabditis elegans.PloS one · 2012Article
- Ca2+ regulates the Drosophila Stoned-A and Stoned-B proteins interaction with the C2B domain of Synaptotagmin-1.PloS one · 2012Article
- Drosophila rolling blackout displays lipase domain-dependent and -independent endocytic functions downstream of dynamin.Traffic (Copenhagen, Denmark) · 2010Article
- SNARE-dependent glutamate release in megakaryocytes.Experimental hematology · 2010Article
- Electrophysiological recording in the Drosophila embryo.Journal of visualized experiments : JoVE · 2009Article
- Harvesting and preparing Drosophila embryos for electrophysiological recording and other procedures.Journal of visualized experiments : JoVE · 2009Article
- Rolling blackout is required for bulk endocytosis in non-neuronal cells and neuronal synapses.Journal of cell science · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Drosophila dicistronic stoned locus encodes two distinctive presynaptic proteins, Stoned A (STNA) and Stoned B (STNB); STNA is a novel protein without homology to known synaptic proteins, and STNB contains a domain with homology to the endocytotic protein AP50. Both Stoned proteins colocalize precisely with endocytotic proteins including the AP2 complex and Dynamin in the "lattice network" characteristic of endocytotic domains in Drosophila presynaptic terminals. FM1-43 dye uptake studies in stoned mutants demonstrate a striking decrease in the size of the endo-exo-cycling synaptic vesicle pool and loss of spatial regulation of the vesicular recycling intermediates. Mutant synapses display a significant delay in vesicular membrane retrieval after depolarization and neurotransmitter release. These studies suggest that the Stoned proteins play a role in mediating synaptic vesicle endocytosis. We have documented previously a highly specific synaptic mislocalization and degradation of Synaptotagmin I in stoned mutants. Here we show that transgenic overexpression of Synaptotagmin I rescues stoned embryonic lethality and restores endocytotic recycling to normal levels. Furthermore, overexpression of Synaptotagmin I in otherwise wild-type animals results in increased synaptic dye uptake, indicating that Synaptotagmin I directly regulates the endo-exo-cycling synaptic vesicle pool size. In parallel with recent biochemical studies, this genetic analysis strongly suggests that Stoned proteins regulate the AP2-Synaptotagmin I interaction during synaptic vesicle endocytosis. We conclude that Stoned proteins control synaptic transmission strength by mediating the retrieval of Synaptotagmin I from the plasma membrane.
Indexed as
Identifiers
11160392PMC6762239W1656523230What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.