ArticleBiophysical journal2001
Computed tomography-based spectral imaging for fluorescence microscopy.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Snapshot hyperspectral imaging microscope enabled by cladded waveguide array fabricated with 2-photon additive manufacturing.Biomedical optics express · 2025Article
- High-spatial density snapshot imaging spectrometer enabled by 2-photon fabricated custom fiber bundles.Optics letters · 2023Article
- Spectral image scanning microscopy.Biomedical optics express · 2019Article
- A review of snapshot multidimensional optical imaging: measuring photon tags in parallel.Physics reports · 2016Article
- Hyperspectral optical tomography of intrinsic signals in the rat cortex.Neurophotonics · 2015Article
- Optical hyperspectral imaging in microscopy and spectroscopy - a review of data acquisition.Journal of biophotonics · 2015Review
- Snapshot 3D optical coherence tomography system using image mapping spectrometry.Optics express · 2013Article
- Quantitative comparison between full-spectrum and filter-based imaging in hyperspectral fluorescence microscopy.Journal of microscopy · 2012Article
- Depth-resolved image mapping spectrometer (IMS) with structured illumination.Optics express · 2011Article
- Real-time snapshot hyperspectral imaging endoscope.Journal of biomedical optics · 2011Article
- Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy.Optics express · 2010Article
- Compact Image Slicing Spectrometer (ISS) for hyperspectral fluorescence microscopy.Optics express · 2009Article
- Lipid bilayer vesicle fusion: intermediates captured by high-speed microfluorescence spectroscopy.Biophysical journal · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The computed tomography imaging spectrometer (CTIS) is a non-scanning instrument capable of simultaneously acquiring full spectral information (450-750 nm) from every position element within its field of view (75 microm x 75 microm). The current spatial and spectral sampling intervals of the spectrometer are 1.0 microm and 10 nm, respectively. This level of resolution is adequate to resolve signal responses from multiple fluorescence probes located within individual cells or different locations within the same cell. Spectral imaging results are presented from the CTIS combined with a commercial inverted fluorescence microscope. Results demonstrate the capability of the CTIS to monitor the spatiotemporal evolution of pH in rat insulinoma cells loaded with SNARF-1. The ability to analyze full spectral information for two-dimensional (x, y) images allows precise evaluation of heterogeneous physiological responses within cell populations. Due to low signal levels, integration times up to 2 s were required. However, reasonable modifications to the instrument design will provide higher system transmission efficiency with increased temporal and spatial resolution. Specifically, a custom optical design including the use of a larger format detector array is under development for a second-generation system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.