ArticleBiophysical journal2001
Quantitative study of electroporation-mediated molecular uptake and cell viability.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
78 citing papers in PubMed, 254 citations in OpenAlex.
- Electrochemotherapy: a new technological approach in treatment of metastases in the liver.Technology in cancer research & treatment · 2011Trial
- [Coupling characteristics of cellular electroporation and electrodeformation based on dynamic Young's modulus: influence of membrane thickness].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2026Article
- Technical safety and efficacy of PFA catheter electrode for airway ablation in a rat model of COPD.Respiratory research · 2026Article
- Spatial Visualization of Secreted Lysyl Oxidase Activity from Single Living Cells via Label-Free Electrochemiluminescence Microscopy.Chemical & biomedical imaging · 2026Article
- Optimizing electroporation via pulse modulation: a molecular dynamics study.European biophysics journal : EBJ · 2025Article
- CRISPR-Cas technologies: Emerging tools from research to clinical application.Journal of microbiology (Seoul, Korea) · 2025Review
- Feasibility Study for the Use of Gene Electrotransfer and Cell Electrofusion as a Single-Step Technique for the Generation of Activated Cancer Cell Vaccines.The Journal of membrane biology · 2024Article
- Titrating chimeric antigen receptors on CAR T cells enabled by a microfluidic-based dosage-controlled intracellular mRNA delivery platform.Biomicrofluidics · 2024Article
- Condition optimization for electroporation transfection in horse skeletal muscle satellite cells.Animal biotechnology · 2024Article
- Efficient electroporation in primary cells with PEDOT:PSS electrodes.Science advances · 2024Article
- A potential paradigm in CRISPR/Cas systems delivery: at the crossroad of microalgal gene editing and algal-mediated nanoparticles.Journal of nanobiotechnology · 2023Review
- Visible Pulsed Laser-Assisted Selective Killing of Cancer Cells with PVP-Capped Plasmonic Gold Nanostars.Micromachines · 2023Article
- Advances in CRISPR Delivery Methods: Perspectives and Challenges.The CRISPR journal · 2022Review
- Modeling and simulation of current-clamp electroporation.Bioelectrochemistry (Amsterdam, Netherlands) · 2022Article
- Microfluidics delivery of DARPP-32 into HeLa cells maintains viability for in-cell NMR spectroscopy.Communications biology · 2022Article
- In Situ Electroporation on PERFECT Filter for High-Efficiency and High-Viability Tumor Cell Labeling.Micromachines · 2022Article
- Electroporation and cell killing by milli- to nanosecond pulses and avoiding neuromuscular stimulation in cancer ablation.Scientific reports · 2022Article
- The cellular response to plasma membrane disruption for nanomaterial delivery.Nano convergence · 2022Review
- High-Throughput and Dosage-Controlled Intracellular Delivery of Large Cargos by an Acoustic-Electric Micro-Vortices Platform.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Ultralong-Time Recovery and Low-Voltage Electroporation for Biological Cell Monitoring Enabled by a Microsized Multipulse Framework.ACS omega · 2021Article
18 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electroporation's use for laboratory transfection and clinical chemotherapy is limited by an incomplete understanding of the effects of electroporation parameters on molecular uptake and cell viability. To address this need, uptake of calcein and viability of DU 145 prostate cancer cells were quantified using flow cytometry for more than 200 different combinations of experimental conditions. The experimental parameters included field strength (0.1-3.3 kV/cm), pulse length (0.05-20 ms), number of pulses (1-10), calcein concentration (10-100 microM), and cell concentration (0.6-23% by volume). These data indicate that neither electrical charge nor energy was a good predictor of electroporation's effects. Instead, both uptake and viability showed a complex dependence on field strength, pulse length, and number of pulses. The effect of cell concentration was explained quantitatively by electric field perturbations caused by neighboring cells. Uptake was shown to vary linearly with external calcein concentration. This large quantitative data set may be used to optimize electroporation protocols, test theoretical models, and guide mechanistic interpretations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.