ArticleBiophysical journal2001
A microcantilever device to assess the effect of force on the lifetime of selectin-carbohydrate bonds.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Examining the Effect of Kindlin-3 Binding Site Mutation on LFA-1-ICAM-1 Bonds by Force Measuring Optical Tweezers.Frontiers in immunology · 2021Article
- Article
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- Heterogeneous and rate-dependent streptavidin-biotin unbinding revealed by high-speed force spectroscopy and atomistic simulations.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Nanoscale Functionalized Particles with Rotation-Controlled Capture in Shear Flow.ACS applied materials & interfaces · 2018Article
- Improving estimation of kinetic parameters in dynamic force spectroscopy using cluster analysis.The Journal of chemical physics · 2018Article
- Intermolecular interactions between glycomodules of plant cell wall arabinogalactan-proteins and extensins.Cell surface (Amsterdam, Netherlands) · 2018Article
- Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling.Molecular biology of the cell · 2018Article
- Micropipette force probe to quantify single-cell force generation: application to T-cell activation.Molecular biology of the cell · 2017Article
- βBiophysical journal · 2017Article
- Viscoelasticity Measurements Reveal Rheological Differences Between Stem-like and Non-stem-like Breast Cancer Cells.Cellular and molecular bioengineering · 2017Article
- Amino acid polymorphisms in the fibronectin-binding repeats of fibronectin-binding protein A affect bond strength and fibronectin conformation.The Journal of biological chemistry · 2017Article
- Nanomechanical and Thermophoretic Analyses of the Nucleotide-Dependent Interactions between the AAA(+) Subunits of Magnesium Chelatase.Journal of the American Chemical Society · 2016Article
- Stochastic simulation of single-molecule pulling experiments.The European physical journal. E, Soft matter · 2014Article
- TNF-α and IFN-γ promote lymphocyte adhesion to endothelial junctional regions facilitating transendothelial migration.Journal of leukocyte biology · 2014Article
- Cytoadherence of erythrocytes invaded by Plasmodium falciparum: quantitative contact-probing of a human malaria receptor.Acta biomaterialia · 2013Article
- Elongated membrane tethers, individually anchored by high affinity α4β1/VCAM-1 complexes, are the quantal units of monocyte arrests.PloS one · 2013Article
- Rupture of single receptor-ligand bonds: a new insight into probability distribution function.Colloids and surfaces. B, Biointerfaces · 2013Article
- A High-Throughput Technique Reveals the Load- and Site Density-Dependent Kinetics of E-Selectin.Cellular and molecular bioengineering · 2012Article
- Single-molecule force spectroscopy of the Aplysia cell adhesion molecule reveals two homophilic bonds.Biophysical journal · 2012Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
Abstract
A microcantilever technique was used to apply force to receptor-ligand molecules involved in leukocyte rolling on blood vessel walls. E-selectin was adsorbed onto 3-microm-diameter, 4-mm-long glass fibers, and the selectin ligand, sialyl Lewis(x), was coupled to latex microspheres. After binding, the microsphere and bound fiber were retracted using a computerized loading protocol that combines hydrodynamic and Hookean forces on the fiber to produce a range of force loading rates (force/time), r(f). From the distribution of forces at failure, the average force was determined and plotted as a function of ln r(f). The slope and intercept of the plot yield the unstressed reverse reaction rate, k(r)(o), and a parameter that describes the force dependence of reverse reaction rates, r(o). The ligand was titrated so adhesion occurred in approximately 30% of tests, implying that >80% of adhesive events involve single bonds. Monte Carlo simulations show that this level of multiple bonding has little effect on parameter estimation. The estimates are r(o) = 0.048 and 0.016 nm and k(r)(o) = 0.72 and 2.2 s(-1) for loading rates in the ranges 200-1000 and 1000-5000 pN s(-1), respectively. Levenberg-Marquardt fitting across all values of r(f) gives r(o) = 0.034 nm and k(r)(o) = 0.82 s(-1). The values of these parameters are in the range required for rolling, as suggested by adhesive dynamics simulations.
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