ArticleThe American journal of pathology2001
Overexpression of matrix metalloproteinase-10 and matrix metalloproteinase-3 in human diabetic corneas: a possible mechanism of basement membrane and integrin alterations.
Article in The American journal of pathology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 113 citations in OpenAlex.
- Safety and efficacy of keratorefractive surgery in diabetes mellitus: A clinical review.World journal of clinical cases · 2026Review
- Shark Cartilage-Derived Anti-Angiogenic Peptide Inhibits Corneal Neovascularization.Bioengineering (Basel, Switzerland) · 2024Article
- Article
- Corneal Epithelial Changes in Diabetic Patients: A Review.International journal of molecular sciences · 2024Review
- Diabetic Keratopathy: Redox Signaling Pathways and Therapeutic Prospects.Antioxidants (Basel, Switzerland) · 2024Review
- Molecular nature of ocular surface barrier function, diseases that affect it, and its relevance for ocular drug delivery.The ocular surface · 2023Review
- Diabetes more than retinopathy, it's effect on the anterior segment of eye.World journal of clinical cases · 2023Review
- Target-driven machine learning-enabled virtual screening (TAME-VS) platform for early-stage hit identification.Frontiers in molecular biosciences · 2023Article
- Gene Therapy in the Anterior Eye Segment.Current gene therapy · 2022Review
- Systemic diseases and the cornea.Experimental eye research · 2021Review
- Novel nanopolymer RNA therapeutics normalize human diabetic corneal wound healing and epithelial stem cells.Nanomedicine : nanotechnology, biology, and medicine · 2021Article
- Expression of integrins to control migration direction of electrotaxis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Overexpression of MMPs in Corneas Requiring Penetrating and Deep Anterior Lamellar Keratoplasty.Investigative ophthalmology & visual science · 2019Article
- Corneal alteration and pathogenesis in diabetes mellitus.International journal of ophthalmology · 2019Review
- Exosomes from normal and diabetic human corneolimbal keratocytes differentially regulate migration, proliferation and marker expression of limbal epithelial cells.Scientific reports · 2018Article
- Diabetic complications in the cornea.Vision research · 2017Review
- Metalloproteinases as mediators of inflammation and the eyes: molecular genetic underpinnings governing ocular pathophysiology.International journal of ophthalmology · 2017Review
- Comparison of the corneal biomechanical properties, optic nerve head topographic parameters, and retinal nerve fiber layer thickness measurements in diabetic and non-diabetic primary open-angle glaucoma.International ophthalmology · 2016Article
- Stromelysin-2 (MMP10) Moderates Inflammation by Controlling Macrophage Activation.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells.Journal of visualized experiments : JoVE · 2016Article
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 3 countries.
Funding
Abstract
We have previously described decreased immunostaining of nidogen-1/entactin; laminin chains alpha1, alpha5, beta1,gamma1; and epithelial integrin alpha3beta1 in human diabetic retinopathy (DR) corneas. Here, using 142 human corneas, we tested whether these alterations might be caused by decreased gene expression levels or increased degradation. By semiquantitative reverse transcription-polymerase chain reaction, gene expression levels of the alpha1, alpha5, and beta1 laminin chains; nidogen-1/entactin; integrin alpha3 and beta1 chains in diabetic and DR corneal epithelium were similar to normal. Thus, the observed basement membrane and integrin changes were unlikely to occur because of a decreased synthesis. mRNA levels of matrix metalloproteinase-10 (MMP-10/stromelysin-2) were significantly elevated in DR corneal epithelium and stroma, and of MMP-3/stromelysin-1, in DR corneal stroma. No such elevation was seen in keratoconus corneas. These data were confirmed by immunostaining, zymography, and Western blotting. mRNA levels of five other proteinases and of three tissue inhibitors of MMPs were similar to normal in diabetic and DR corneal epithelium and stroma. The data suggest that alterations of laminins, nidogen-1/entactin, and epithelial integrin in DR corneas may occur because of an increased proteolytic degradation. MMP-10 overexpressed in the diabetic corneal epithelium seems to be the major contributor to the observed changes in DR corneas. Such alterations may bring about epithelial adhesive abnormalities clinically seen in diabetic corneas.
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Registered trials
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