ArticleProceedings of the National Academy of Sciences of the United States of America2001
Caspase-2 pre-mRNA alternative splicing: Identification of an intronic element containing a decoy 3' acceptor site.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 67 citations in OpenAlex.
- Structural basis for specific RNA recognition by the alternative splicing factor RBM5.Nature communications · 2023Article
- SRSF9 Regulates Cassette Exon Splicing of Caspase-2 by Interacting with Its Downstream Exon.Cells · 2021Article
- TCF3 mutually exclusive alternative splicing is controlled by long-range cooperative actions between hnRNPH1 and PTBP1.RNA (New York, N.Y.) · 2019Article
- An important class of intron retention events in human erythroblasts is regulated by cryptic exons proposed to function as splicing decoys.RNA (New York, N.Y.) · 2018Article
- From General Aberrant Alternative Splicing in Cancers and Its Therapeutic Application to the Discovery of an Oncogenic DMTF1 Isoform.International journal of molecular sciences · 2017Review
- A dynamic intron retention program enriched in RNA processing genes regulates gene expression during terminal erythropoiesis.Nucleic acids research · 2016Article
- Staufen1 Regulates Multiple Alternative Splicing Events either Positively or Negatively in DM1 Indicating Its Role as a Disease Modifier.PLoS genetics · 2016Article
- Stress-induced endogenous siRNAs targeting regulatory intron sequences in Brachypodium.RNA (New York, N.Y.) · 2015Article
- Exon 9 skipping of apoptotic caspase-2 pre-mRNA is promoted by SRSF3 through interaction with exon 8.Biochimica et biophysica acta · 2014Article
- Rbfox proteins regulate alternative mRNA splicing through evolutionarily conserved RNA bridges.Nature structural & molecular biology · 2013Article
- The RNA-binding protein Staufen1 is increased in DM1 skeletal muscle and promotes alternative pre-mRNA splicing.The Journal of cell biology · 2012Article
- Caspases and cancer.Cell death and differentiation · 2011Review
- Alternative pre-mRNA splicing regulation in cancer: pathways and programs unhinged.Genes & development · 2010Review
- Deciphering the splicing code.Nature · 2010Article
- The intronic splicing code: multiple factors involved in ATM pseudoexon definition.The EMBO journal · 2010Article
- Dynamic regulation of alternative splicing by silencers that modulate 5' splice site competition.Cell · 2008Article
- Up-regulation of the proapoptotic caspase 2 splicing isoform by a candidate tumor suppressor, RBM5.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Polypyrimidine tract-binding protein (PTB) differentially affects malignancy in a cell line-dependent manner.The Journal of biological chemistry · 2008Article
- Article
- Identification of photoreceptor genes affected by PRPF31 mutations associated with autosomal dominant retinitis pigmentosa.Neurobiology of disease · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
We have established a model system using the caspase-2 pre-mRNA and initiated a study on the role of alternative splicing in regulation of programmed cell death. A caspase-2 minigene construct has been made that can be alternatively spliced in transfected cells and in nuclear extracts. Using this system, we have identified a 100-nt region in downstream intron 9 that inhibits the inclusion of the 61-bp alternative exon. This element (In100) can facilitate exon skipping in the context of competing 3' or 5' splice sites, but not in single-intron splicing units. The In100 element is also active in certain heterologous pre-mRNAs, although in a highly context-dependent manner. Interestingly, we found that In100 contains a sequence that highly resembles a bona fide 3' splice site. We provide evidence that this sequence acts as a "decoy" acceptor site that engages in U2 snRNP-dependent but nonproductive splicing complexes with the 5' splice site of exon 9, hence conferring competitive advantage to the exon-skipping splicing event (E8-E10). These results reveal a mechanism of action for a negative intronic regulatory element and uncover a role for U2 snRNP in the regulation of alternative splicing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.