ArticleProceedings of the National Academy of Sciences of the United States of America2001
Ribosome shunt is essential for infectivity of cauliflower mosaic virus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Poly(U)-specific endoribonuclease ENDOU promotes translation of human CHOP mRNA by releasing uORF element-mediated inhibition.The EMBO journal · 2021Article
- Ribosome Shunting, Polycistronic Translation, and Evasion of Antiviral Defenses in Plant Pararetroviruses and Beyond.Frontiers in microbiology · 2018Review
- uORFdb--a comprehensive literature database on eukaryotic uORF biology.Nucleic acids research · 2014Article
- How can plant DNA viruses evade siRNA-directed DNA methylation and silencing?International journal of molecular sciences · 2013Review
- Review
- Short ORF-dependent ribosome shunting operates in an RNA picorna-like virus and a DNA pararetrovirus that cause rice tungro disease.PLoS pathogens · 2012Article
- Massive production of small RNAs from a non-coding region of Cauliflower mosaic virus in plant defense and viral counter-defense.Nucleic acids research · 2011Article
- Mechanisms employed by retroviruses to exploit host factors for translational control of a complicated proteome.Retrovirology · 2009Review
- Virus versus host cell translation love and hate stories.Advances in virus research · 2009Review
- Gaussia-luciferase as a sensitive reporter gene for monitoring promoter activity in the nucleus of the green alga Chlamydomonas reinhardtii.Molecular genetics and genomics : MGG · 2008Article
- Article
- Both linear and discontinuous ribosome scanning are used for translation initiation from bicistronic human immunodeficiency virus type 1 env mRNAs.Journal of virology · 2007Article
- Mechanism of ribosome shunting in Rice tungro bacilliform pararetrovirus.RNA (New York, N.Y.) · 2006Article
- Tethering of eIF4G to adenoviral mRNAs by viral 100k protein drives ribosome shunting.Genes & development · 2004Article
- A distinct endogenous pararetrovirus family in Nicotiana tomentosiformis, a diploid progenitor of polyploid tobacco.Plant physiology · 2004Article
- 5'-untranslated regions with multiple upstream AUG codons can support low-level translation via leaky scanning and reinitiation.Nucleic acids research · 2004Article
- Identification of a cis-acting element required for shunt-mediated translational initiation of the Sendai virus Y proteins.Nucleic acids research · 2003Article
- Viral strategies of translation initiation: ribosomal shunt and reinitiation.Progress in nucleic acid research and molecular biology · 2002Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cauliflower mosaic virus (CaMV) is a DNA-containing pararetrovirus replicating by means of reverse transcription of a terminally redundant pregenomic 35S RNA that is also used as a polycistronic mRNA. The leader of 35S RNA is long, highly structured, and contains multiple short ORFs (sORFs), which strongly interfere with the ribosome scanning process. Translation of this RNA is initiated by a ribosome shunt mechanism, in which ribosomes translate the most 5'-proximal short ORF (sORF A), then skip a large region of the leader containing a putative RNA encapsidation signal and reinitiate translation at the first long viral ORF. Here, we demonstrate that the efficiency of the sORF A-mediated ribosome shunt is an important determinant of viral infectivity. Point mutations in sORF A, which reduced the basal level of shunt-dependent expression and the degree of shunt enhancement by a CaMV-encoded translation transactivator (TAV), consequently reduced infectivity of the virus in turnip plants. First- or second-site reversions appeared in the viral progeny. The second-site reversions restored shuntdependent expression to an extent correlating with their relative abundance in the progeny. Mutations that abolished both the basal and TAV-activated components of shunting proved to be lethal. Finally, by using an artificial stem structure that blocks scanning, we obtained direct evidence that ribosome shunt operates during CaMV infection.
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