ArticleThe Plant cell2001
Downregulation of caffeic acid 3-O-methyltransferase and caffeoyl CoA 3-O-methyltransferase in transgenic alfalfa. impacts on lignin structure and implications for the biosynthesis of G and S lignin.
Article in The Plant cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 181 papers.
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Who cites it
181 citing papers in PubMed, 514 citations in OpenAlex.
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- MsCCoAOMTh3 confers drought tolerance by mediating lignin content and ROS scavenging.Plant molecular biology · 2026Article
- Genome-wide identification of CAD and CCoAOMT gene families in soybean and analysis of expression patterns underFrontiers in plant science · 2026Article
- Genome-wide association study of a Guinea grass (Megathyrsus maximus) diversity panel reveals the genetic basis of agronomic and nutritional traits.BMC plant biology · 2025Article
- Integrated analysis of transcriptome and metabolome reveals the mechanism of lignin biosynthesis in fruit abscission of Chinese bayberry (Myrica rubra).BMC plant biology · 2025Article
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- Using transcriptome analysis and QTL mapping to decipher the genetic basis of heterosis in potato root system.BMC plant biology · 2025Article
- Lignin: the primary component responsible for endocarp sclerosis in the development of Cornus officinalis fruit.BMC plant biology · 2025Article
- Forage Crop Research in the Modern Age.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Integrative analyses reveal Bna-miR397a-BnaLAC2 as a potential modulator of low-temperature adaptability in Brassica napus L.Plant biotechnology journal · 2025Article
- Proteome Reprogramming and Acquired Stress Tolerance in Potato Cells Exposed to Acute or Stepwise Water Deficit.Plant, cell & environment · 2025Article
- Transcriptomics-proteomics analysis reveals StCOMT1 regulates drought, alkali and combined stresses in potato.Plant cell reports · 2025Article
- A tale for two roles: Root-secreted methyl ferulate inhibits P. nicotianae and enriches the rhizosphere Bacillus against black shank disease in tobacco.Microbiome · 2025Article
- The cell colony development is connected with the accumulation of embryogenesis-related proteins and dynamic distribution of cell wall components in in vitro cultures of Fagopyrum tataricum and Fagopyrum esculentum.BMC plant biology · 2025Article
- Metabolic and transcriptional analysis of tuber expansion in Curcuma kwangsiensis.Scientific reports · 2025Article
- Mechanism of nodulation and nitrogen fixation in Caucasian clover (Frontiers in genetics · 2025Article
- Functional characterization of COMT genes in Chinese white pear (Frontiers in plant science · 2025Article
121 more citing papers are in PubMed but not listed here.
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5 authors at 1 institution in 1 country.
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Abstract
Transgenic alfalfa plants were generated harboring caffeic acid 3-O-methyltransferase (COMT) and caffeoyl CoA 3-O-methyltransferase (CCOMT) cDNA sequences under control of the bean phenylalanine ammonia-lyase PAL2 promoter. Strong downregulation of COMT resulted in decreased lignin content, a reduction in total guaiacyl (G) lignin units, a near total loss of syringyl (S) units in monomeric and dimeric lignin degradation products, and appearance of low levels of 5-hydroxy guaiacyl units and a novel dimer. No soluble monolignol precursors accumulated. In contrast, strong downregulation of CCOMT led to reduced lignin levels, a reduction in G units without reduction in S units, and increases in beta-5 linked dimers of G units. Accumulation of soluble caffeic acid beta-d-glucoside occurred only in CCOMT downregulated plants. The results suggest that CCOMT does not significantly contribute to the 3-O-methylation step in S lignin biosynthesis in alfalfa and that there is redundancy with respect to the 3-O-methylation reaction of G lignin biosynthesis. COMT is unlikely to catalyze the in vivo methylation of caffeic acid during lignin biosynthesis.
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