ArticleMolecular and cellular biology2001
Distinctive features of Drosophila alternative splicing factor RS domain: implication for specific phosphorylation, shuttling, and splicing activation.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- TET dioxygenases localize at splicing speckles and promote RNA splicing.Nucleus (Austin, Tex.) · 2025Article
- The RasGAP-associated endoribonuclease G3BP mediates stress granule assembly.The Journal of cell biology · 2023Article
- Article
- A novel Plasmodium falciparum SR protein is an alternative splicing factor required for the parasites' proliferation in human erythrocytes.Nucleic acids research · 2012Article
- Protein kinase a-dependent phosphorylation of serine 119 in the proto-oncogenic serine/arginine-rich splicing factor 1 modulates its activity as a splicing enhancer protein.Genes & cancer · 2011Article
- A Drosophila model of FUS-related neurodegeneration reveals genetic interaction between FUS and TDP-43.Human molecular genetics · 2011Article
- DX16 is a novel SR protein phosphorylated by DOA.Molecular and cellular biochemistry · 2008Article
- Small-molecule inhibition of HIV pre-mRNA splicing as a novel antiretroviral therapy to overcome drug resistance.PLoS pathogens · 2007Article
- The maize zmsmu2 gene encodes a putative RNA-splicing factor that affects protein synthesis and RNA processing during endosperm development.Plant physiology · 2007Article
- The SR family proteins B52 and dASF/SF2 modulate development of the Drosophila visual system by regulating specific RNA targets.Molecular and cellular biology · 2007Article
- Eye development under the control of SRp55/B52-mediated alternative splicing of eyeless.PloS one · 2007Article
- Phosphorylation status of the Kep1 protein alters its affinity for its protein binding partner alternative splicing factor ASF/SF2.The Biochemical journal · 2006Article
- Negative feedback regulation among SR splicing factors encoded by Rbp1 and Rbp1-like in Drosophila.The EMBO journal · 2005Article
- Selective modification of alternative splicing by indole derivatives that target serine-arginine-rich protein splicing factors.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- p210BCR/ABL-induced alteration of pre-mRNA splicing in primary human CD34+ hematopoietic progenitor cells.Leukemia · 2004Article
- Nuclear bodies and compartmentalization of pre-mRNA splicing factors in higher plants.Chromosoma · 2004Article
- Alternative splicing modulation by a LAMMER kinase impinges on developmental and transcriptome expression.The Plant cell · 2003Article
- The RasGAP-associated endoribonuclease G3BP assembles stress granules.The Journal of cell biology · 2003Article
- Nuclear export and retention signals in the RS domain of SR proteins.Molecular and cellular biology · 2002Article
- A conserved Drosophila transportin-serine/arginine-rich (SR) protein permits nuclear import of Drosophila SR protein splicing factors and their antagonist repressor splicing factor 1.Molecular biology of the cell · 2002Article
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7 authors.
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Abstract
The human splicing factor 2, also called human alternative splicing factor (hASF), is the prototype of the highly conserved SR protein family involved in constitutive and regulated splicing of metazoan mRNA precursors. Here we report that the Drosophila homologue of hASF (dASF) lacks eight repeating arginine-serine dipeptides at its carboxyl-terminal region (RS domain), previously shown to be important for both localization and splicing activity of hASF. While this difference has no effect on dASF localization, it impedes its capacity to shuttle between the nucleus and cytoplasm and abolishes its phosphorylation by SR protein kinase 1 (SRPK1). dASF also has an altered splicing activity. While being competent for the regulation of 5' alternative splice site choice and activation of specific splicing enhancers, dASF fails to complement S100-cytoplasmic splicing-deficient extracts. Moreover, targeted overexpression of dASF in transgenic flies leads to higher deleterious developmental defects than hASF overexpression, supporting the notion that the distinctive structural features at the RS domain between the two proteins are likely to be functionally relevant in vivo.
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