ArticleMolecular and cellular biology2001
Stability of a human SWI-SNF remodeled nucleosomal array.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Collaboration through chromatin: motors of transcription and chromatin structure.Journal of molecular biology · 2021Review
- Distortion of histone octamer core promotes nucleosome mobilization by a chromatin remodeler.Science (New York, N.Y.) · 2017Article
- Mechanisms of ATP-Dependent Chromatin Remodeling Motors.Annual review of biophysics · 2016Review
- Regulation of DNA Translocation Efficiency within the Chromatin Remodeler RSC/Sth1 Potentiates Nucleosome Sliding and Ejection.Molecular cell · 2016Article
- Decoupling nucleosome recognition from DNA binding dramatically alters the properties of the Chd1 chromatin remodeler.Nucleic acids research · 2013Article
- Mechanism(s) of SWI/SNF-induced nucleosome mobilization.Chembiochem : a European journal of chemical biology · 2011Review
- Human SWI/SNF drives sequence-directed repositioning of nucleosomes on C-myc promoter DNA minicircles.Biochemistry · 2007Article
- Human SWI/SNF generates abundant, structurally altered dinucleosomes on polynucleosomal templates.Molecular and cellular biology · 2005Article
- Using atomic force microscopy to study nucleosome remodeling on individual nucleosomal arrays in situ.Biophysical journal · 2004Article
- High-resolution mapping of changes in histone-DNA contacts of nucleosomes remodeled by ISW2.Molecular and cellular biology · 2002Article
- Nucleosome remodeling by the human SWI/SNF complex requires transient global disruption of histone-DNA interactions.Molecular and cellular biology · 2002Article
- Direct imaging of human SWI/SNF-remodeled mono- and polynucleosomes by atomic force microscopy employing carbon nanotube tips.Molecular and cellular biology · 2001Article
- Transcriptional activation domains of human heat shock factor 1 recruit human SWI/SNF.Molecular and cellular biology · 2001Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SWI-SNF alters DNA-histone interactions within a nucleosome in an ATP-dependent manner. These alterations cause changes in the topology of a closed circular nucleosomal array that persist after removal of ATP from the reaction. We demonstrate here that a remodeled closed circular array will revert toward its original topology when ATP is removed, indicating that the remodeled array has a higher energy than that of the starting state. However, reversion occurs with a half-life measured in hours, implying a high energy barrier between the remodeled and standard states. The addition of competitor DNA accelerates reversion of the remodeled array by more than 10-fold, and we interpret this result to mean that binding of human SWI-SNF (hSWI-SNF), even in the absence of ATP hydrolysis, stabilizes the remodeled state. In addition, we also show that SWI-SNF is able to remodel a closed circular array in the absence of topoisomerase I, demonstrating that hSWI-SNF can induce topological changes even when conditions are highly energetically unfavorable. We conclude that the remodeled state is less stable than the standard state but that the remodeled state is kinetically trapped by the high activation energy barrier separating it from the unremodeled conformation.
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