ArticleMolecular and cellular biology2001
Intercistronic region required for polycistronic pre-mRNA processing in Caenorhabditis elegans.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 60 citations in OpenAlex.
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- phiC31 integrase for recombination-mediated single-copy insertion and genome manipulation in Caenorhabditis elegans.Genetics · 2022Article
- An improved in vivo tethering assay with single molecule FISH reveals that a nematode Nanos enhances reporter expression and mRNA stability.RNA (New York, N.Y.) · 2021Article
- Dynamics of Notch-Dependent Transcriptional Bursting in Its Native Context.Developmental cell · 2019Article
- Developmental dynamics of gene expression and alternative polyadenylation in the Caenorhabditis elegans germline.Genome biology · 2018Article
- Two distinct transcription termination modes dictated by promoters.Genes & development · 2017Article
- A toolkit for GFP-mediated tissue-specific protein degradation inDevelopment (Cambridge, England) · 2017Article
- Sexually dimorphic control of gene expression in sensory neurons regulates decision-making behavior ineLife · 2017Article
- A multispecies polyadenylation site model.BMC bioinformatics · 2013Article
- Trans-splicing and operons in C. elegans.WormBook : the online review of C. elegans biology · 2012Review
- Incorporation of a horizontally transferred gene into an operon during cnidarian evolution.PloS one · 2012Article
- Distinct mechanisms for delimiting expression of four Caenorhabditis elegans transcription factor genes encoding activators or repressors.Molecular genetics and genomics : MGG · 2011Article
- Identification of transcription start sites of trans-spliced genes: uncovering unusual operon arrangements.RNA (New York, N.Y.) · 2011Article
- Polycistronic pre-mRNA processing in vitro: snRNP and pre-mRNA role reversal in trans-splicing.Genes & development · 2010Article
- Alternative trans-splicing of Caenorhabditis elegans sma-9/schnurri generates a short transcript that provides tissue-specific function in BMP signaling.BMC molecular biology · 2010Article
- Functional analysis of putative operons in Brugia malayi.International journal for parasitology · 2010Article
- Regulation of transcription termination in the nematode Caenorhabditis elegans.Nucleic acids research · 2009Article
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6 authors at 1 institution in 1 country.
Funding
Abstract
In Caenorhabditis elegans, polycistronic pre-mRNAs are processed by cleavage and polyadenylation at the 3' ends of the upstream genes and trans splicing, generally to the specialized spliced leader SL2, at the 5' ends of the downstream genes. Previous studies have indicated a relationship between these two events in the processing of a heat shock-induced gpd-2-gpd-3 polycistronic pre-mRNA. Here, we report mutational analysis of the intercistronic region of this operon by linker scan analysis. Surprisingly, no sequences downstream of the 3' end were important for 3'-end formation. In contrast, a U-rich (Ur) element located 29 bp downstream of the site of 3'-end formation was shown to be important for downstream mRNA biosynthesis. This approximately 20-bp element is sufficient for SL2 trans splicing and mRNA accumulation when transplanted to a heterologous context. Furthermore, when the downstream gene was replaced by a gene from another organism, no loss of trans-splicing specificity was observed, suggesting that the Ur element may be the primary signal required for downstream mRNA processing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.