ArticleMolecular and cellular biology2001
The Chinese hamster dihydrofolate reductase replication origin beta is active at multiple ectopic chromosomal locations and requires specific DNA sequence elements for activity.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 81 citations in OpenAlex.
- Origins of DNA replication in eukaryotes.Molecular cell · 2023Review
- Nucleoporins facilitate ORC loading onto chromatin.Cell reports · 2022Article
- The genetic architecture of DNA replication timing in human pluripotent stem cells.Nature communications · 2021Observational
- The hunt for origins of DNA replication in multicellular eukaryotes.F1000prime reports · 2015Review
- Exonuclease mutations in DNA polymerase epsilon reveal replication strand specific mutation patterns and human origins of replication.Genome research · 2014Article
- Chromatin structure and replication origins: determinants of chromosome replication and nuclear organization.Journal of molecular biology · 2014Review
- Allele-specific genome-wide profiling in human primary erythroblasts reveal replication program organization.PLoS genetics · 2014Article
- Activation of a human chromosomal replication origin by protein tethering.Nucleic acids research · 2013Article
- Article
- Initiation of DNA Replication in the Human Genome.Hereditary genetics : current research · 2012Article
- The dyad symmetry element of Epstein-Barr virus is a dominant but dispensable replication origin.PloS one · 2011Article
- Structural diversity and dynamics of genomic replication origins in Schizosaccharomyces pombe.The EMBO journal · 2010Article
- A winding road to origin discovery.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Discrete functional elements required for initiation activity of the Chinese hamster dihydrofolate reductase origin beta at ectopic chromosomal sites.Experimental cell research · 2007Article
- Differential binding of replication proteins across the human c-myc replicator.Molecular and cellular biology · 2006Article
- Mapping of DNA replication origins to noncoding genes of the X-inactivation center.Molecular and cellular biology · 2006Article
- DNA bending in the replication zone of the C3 DNA puff amplicon of Rhynchosciara americana (Diptera: Sciaridae).Molecular biology reports · 2006Article
- Essential elements of a licensed, mammalian plasmid origin of DNA synthesis.Molecular and cellular biology · 2006Article
- Temporal profile of replication of human chromosomes.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Specific signals at the 3' end of the DHFR gene define one boundary of the downstream origin of replication.Genes & development · 2005Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
To identify cis-acting genetic elements essential for mammalian chromosomal DNA replication, a 5.8-kb fragment from the Chinese hamster dihydrofolate reductase (DHFR) locus containing the origin beta (ori-beta) initiation region was stably transfected into random ectopic chromosomal locations in a hamster cell line lacking the endogenous DHFR locus. Initiation at ectopic ori-beta in uncloned pools of transfected cells was measured using a competitive PCR-based nascent strand abundance assay and shown to mimic that at the endogenous ori-beta region in Chinese hamster ovary K1 cells. Initiation activity of three ectopic ori-beta deletion mutants was reduced, while the activity of another deletion mutant was enhanced. The results suggest that a 5.8-kb fragment of the DHFR ori-beta region is sufficient to direct initiation and that specific DNA sequences in the ori-beta region are required for efficient initiation activity.
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