Evidence map›Paper›PMID 11158125›Full record

ArticleJournal of clinical microbiology2001

Cloning and characterization of a complex DNA fingerprinting probe for Candida parapsilosis.

L Enger, S Joly, C Pujol, P Simonson, M Pfaller, D R Soll

Abstract read
In one paragraph

Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Journal of fungi (Basel, Switzerland) · 2021
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  9. MALDI-TOF mass spectrometry and microsatellite markers to evaluate Candida parapsilosis transmission in neonatal intensive care units.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012
    Article
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  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

L EngerDepartment of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA.
S Joly
C Pujol
P Simonson
M Pfaller
D R Soll

Funding

SWITCHING AND CANDIDA PATHOGENESIS--A MOLECULAR ANALYSISR01AI039735 · NIAID · UNIVERSITY OF IOWA · PI SOLL, DAVID R. · 1996 to 2005
$1.5M
ROLE OF ANTIOXIDANT ENZYMES IN ORAL CANCERP50DE010758 · NIDCR · UNIVERSITY OF IOWA · PI SWARTZENDRUBER, DONALD C · 1993 to 1998
–
NIAID NIH HHS AI39735NIAID NIH HHS R01 AI039735NIDCR NIH HHS DE10758
6 · The paper itself

Abstract

Candida parapsilosis accounts for a significant number of nosocomial fungemias, but in fact, no effective and verified genetic fingerprinting method has emerged for assessing the relatedness of independent isolates for epidemiological studies. A complex 15-kb DNA fingerprinting probe, Cp3-13, was therefore isolated from a library of C. parapsilosis genomic DNA fragments. The efficacy of Cp3-13 for DNA fingerprinting was verified by a comparison of its clustering capacity with those of randomly amplified polymorphic DNA analysis and internally transcribed spacer region sequencing, by testing species specificity, and by assessing its capacity to identify microevolutionary changes both in vitro and in vivo. Southern blot hybridization of EcoRI/SalI-digested DNA with Cp3-13 provides a fingerprinting system that (i) identifies the same strain in independent isolates, (ii) discriminates between unrelated isolates, (iii) separates independent isolates into valid groups in a dendrogram, (iv) identifies microevolution in infecting populations, and (v) is amenable to automatic computer-assisted DNA fingerprint analysis. This probe is now available for epidemiological studies.

Indexed as

PhylogenyCandidaCandidiasisCloning, MolecularDeoxyribonuclease EcoRIDNA, BacterialDNA FingerprintingDNA ProbesHumansRandom Amplified Polymorphic DNA TechniqueRestriction MappingDeoxyribonuclease EcoRIDNA, BacterialDNA Probes

Identifiers

PMID11158125
PMCPMC87794

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.