ArticleJournal of clinical microbiology2001
Consensus and variable region PCR analysis of Helicobacter pylori 3' region of cagA gene in isolates from individuals with or without peptic ulcer.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 46 citations in OpenAlex.
- Article
- Diversity of 3' variable region of cagA gene in Helicobacter pylori strains isolated from Chinese population.Gut pathogens · 2021Article
- Evaluation of the Pattern of EPIYA Motifs in the Helicobacter pylori cagA Gene of Patients with Gastritis and Gastric Adenocarcinoma from the Brazilian Amazon Region.International journal of bacteriology · 2014Article
- Evaluation of diagnostic methods for the detection of Helicobacter pylori in gastric biopsy specimens of dyspeptic patients.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2012Article
- Distinct repeat motifs at the C-terminal region of CagA of Helicobacter pylori strains isolated from diseased patients and asymptomatic individuals in West Bengal, India.Gut pathogens · 2012Article
- Virulence attributes of Helicobacter pylori isolates & their association with gastroduodenal disease.The Indian journal of medical research · 2011Article
- Molecular Basis of pathogenicity in Helicobacter pylori clinical isolates.Journal of clinical microbiology · 2010Article
- Role of innate immunity in Helicobacter pylori-induced gastric malignancy.Physiological reviews · 2010Review
- Detection of Helicobacter pylori by phenotypic and genotypic methods.Digestive diseases and sciences · 2010Article
- Surreptitious manipulation of the human host by Helicobacter pylori.Gut microbes · 2010Article
- Application of PCR amplicon sequencing using a single primer pair in PCR amplification to assess variations in Helicobacter pylori CagA EPIYA tyrosine phosphorylation motifs.BMC research notes · 2010Article
- A comprehensive sequence and disease correlation analyses for the C-terminal region of CagA protein of Helicobacter pylori.PloS one · 2009Article
- Role of Helicobacter pylori CagA molecular variations in induction of host phenotypes with carcinogenic potential.The Journal of infectious diseases · 2009Article
- Helicobacter pylori genotypes identified in gastric biopsy specimens from Jordanian patients.BMC gastroenterology · 2006Article
- A new subtype of 3' region of cagA gene in Helicobacter pylori strains isolated from Zhejiang Province in China.World journal of gastroenterology · 2004Article
- Analysis of Helicobacter pylori genotypes and correlation with clinical outcome in Turkey.Journal of clinical microbiology · 2004Article
- Clarifications regarding the 3' repeat region of the cagA gene in Helicobacter pylori and clinical outcome.Journal of clinical microbiology · 2001Article
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4 authors at 3 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
The clinical outcome of Helicobacter pylori infection may be associated with the cagA bacterial genotype. To investigate the cagA status of H. pylori-infected patients and the relationship between cagA and peptic ulcer disease, gastric biopsy specimens from 103 Caucasian patients in Brazil were analyzed by PCR. Since allelic variation in cagA exists and distinct H. pylori subgenotypes may circulate in different regions, PCR using primers for a variable 3' region of the cagA gene according to a Japanese methodology and for a consensus cagA 3' region used in Western methods was used for cagA detection. cagA was present in 53 (71%) of 75 H. pylori-positive cases when analyzed by the consensus region method and was associated with duodenal ulcer disease (P = 0.02), but not with gastric ulcer (P = 0.26), when compared to patients with duodenitis or gastritis. The variable region PCR method was able to detect 43 (57%) cagA-positive cases within the same group of H. pylori-positive patients and showed three subtypes of cagA (A, B/D, and C) that were not associated with clinical outcome. However, in 8 (18%) of the cases, more than one subtype was present, and an association between patients with multiple subtypes and disease outcome was observed when compared to patients with isolated subtypes (P = 0.048). cagA was a marker of H. pylori strains for duodenal ulcer disease in our population, and in spite of the differences in the 3' region of the cagA gene, the Japanese methodology was able to detect the cagA status in most cases. The presence of multiple subgenotypes of cagA was associated with gastric ulcer.
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