ArticleJournal of clinical microbiology2001
Rapid typing of human adenoviruses by a general PCR combined with restriction endonuclease analysis.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 140 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
140 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Systematic review of point-of-need molecular diagnostics for rotavirus and enteric adenoviruses F40/F41.BMC infectious diseases · 2026Pooled it
- Viral genotype and the pace of epidemic waves: an assessment from SARS-CoV 2 genomics and wastewater surveillance data.Archives of virology · 2026Article
- Development of a Rapid Isothermal Assay for Detection of Adenovirus Types Important in Respiratory Infections.Influenza and other respiratory viruses · 2025Article
- Comparative evaluation of the virucidal activities of ozonated water, povidone-iodine, and ethanol against human adenovirus.Journal of water and health · 2025Article
- Utilizing Viral Metagenomics to Characterize Pathogenic and Commensal Viruses in Pediatric Patients with Febrile Neutropenia.Viruses · 2025Article
- Evaluation of shotgun metagenomics as a diagnostic tool for infectious gastroenteritis.PloS one · 2025Article
- Viral Metagenomics in Patients Who Underwent Allogeneic Hematopoietic Stem Cell Transplantation (HSCT): A Brazilian Experience.Microorganisms · 2024Article
- Evaluation of different genomic regions of rotavirus B and rotavirus C for development of real-time RT‒PCR assays.Virology journal · 2024Article
- Assessment of Gastroenteric Viruses in Marketed Bivalve Mollusks in the Tourist Cities of Rio de Janeiro, Brazil, 2022.Viruses · 2024Article
- Human adenovirus-associated health risk in the recreational waters of the Yal-ku lagoon in the Mexican Caribbean.Journal of water and health · 2024Article
- Molecular analysis of adenovirus strains responsible for gastroenteritis in children, under five, in Tunisia.Heliyon · 2024Article
- Article
- Investigation of Human and Animal Viruses in Water Matrices from a Rural Area in Southeastern Region of Brazil and Their Potential Use as Microbial Source-Tracking Markers.Food and environmental virology · 2023Article
- Sequencing directly from antigen-detection rapid diagnostic tests in Belgium, 2022: a gamechanger in genomic surveillance?Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2023Article
- Review
- Article
- Epidemiology of Human Adenovirus in Pakistani Children Hospitalized with Community-Acquired Gastroenteritis under the Age of Five Years.International journal of environmental research and public health · 2022Article
- Human enteric adenovirus F40/41 as a major cause of acute gastroenteritis in children in Brazil, 2018 to 2020.Scientific reports · 2022Article
- Adenovirus is prevalent in juvenile polyps and correlates with low vitamin D receptor expression.Pediatric research · 2022Article
- Surveillance of Adenovirus and Norovirus Contaminants in the Water and Shellfish of Major Oyster Breeding Farms and Fishing Ports in Taiwan.Pathogens (Basel, Switzerland) · 2022Article
80 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a system for rapid typing of adenoviruses (Ads) based on a combination of PCR and restriction endonuclease (RE) digestion (PCR-RE digestion). Degenerated consensus primers were designed, allowing amplification of DNA from all 51 human Ad prototype strains and altogether 44 different genome variants of Ad serotypes 1, 3, 4, 5, 7, 11, 19, 40, and 41. The 301-bp amplimer of 22 prototype strains representing all six subgenera and the genome variant was selected as a target for sequencing to look for subgenus and genome type variabilities. The sequences obtained were used to facilitate the selection of specific REs for discrimination purposes in a diagnostic assay by following the concept of cleavage or noncleavage of the 301-bp amplimer. On the basis of these results, a flowchart was constructed, allowing identification of subgenus B:2 and D serotypes and almost complete distinction of subgenus A, B:1, C, E, and F serotypes. Application of the PCR-RE digestion system to clinical samples allowed typing of 34 of 40 clinical samples positive for Ad. The genome type determined by this method was identical to that obtained by traditional RE typing of full-length Ad DNA. The remaining six samples were positive only after a nested PCR. Therefore, to reduce the risk of false-negative results, samples scored negative by the PCR-RE digestion system should be evaluated by the described nested PCR. Used in combination, the PCR-RE digestion method and the nested PCR provide a reliable and sensitive system that can easily be applied to all kinds of clinical samples when rapid identification of adenoviruses is needed.
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