ArticleThe Journal of cell biology2001
Tortoise, a novel mitochondrial protein, is required for directional responses of Dictyostelium in chemotactic gradients.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 44 citations in OpenAlex.
- Dynamics of Actin Cytoskeleton and Their Signaling Pathways during Cellular Wound Repair.Cells · 2022Article
- Collective cell migration ofCommunications biology · 2019Article
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
- Specific growth suppression of human cancer cells by targeted delivery of Dictyostelium mitochondrial ribosomal protein S4.Cancer cell international · 2014Article
- Control of cell differentiation by mitochondria, typically evidenced in dictyostelium development.Biomolecules · 2013Article
- Ndufaf5 deficiency in the Dictyostelium model: new roles in autophagy and development.Molecular biology of the cell · 2013Article
- Expression of actin-interacting protein 1 suppresses impaired chemotaxis of Dictyostelium cells lacking the Na+-H+ exchanger NHE1.Molecular biology of the cell · 2010Article
- Dictyostelium discoideum--a model for many reasons.Molecular and cellular biochemistry · 2009Review
- A protein with similarity to PTEN regulates aggregation territory size by decreasing cyclic AMP pulse size during Dictyostelium discoideum development.Eukaryotic cell · 2008Article
- CnrN regulates Dictyostelium group size using a counting factor-independent mechanism.Communicative & integrative biology · 2008Article
- Polyphosphate kinase 1, a conserved bacterial enzyme, in a eukaryote, Dictyostelium discoideum, with a role in cytokinesis.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- PLA2 and PI3K/PTEN pathways act in parallel to mediate chemotaxis.Developmental cell · 2007Article
- Two Dictyostelium orthologs of the prokaryotic cell division protein FtsZ localize to mitochondria and are required for the maintenance of normal mitochondrial morphology.Eukaryotic cell · 2003Article
- Chaperonin 60 and mitochondrial disease in Dictyostelium.Journal of muscle research and cell motility · 2002Article
- Mitochondrial membrane dynamics are altered in cluA- mutants of Dictyostelium.Journal of muscle research and cell motility · 2002Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We have identified a novel gene, Tortoise (TorA), that is required for the efficient chemotaxis of Dictyostelium discoideum cells. Cells lacking TorA sense chemoattractant gradients as indicated by the presence of periodic waves of cell shape changes and the localized translocation of cytosolic PH domains to the membrane. However, they are unable to migrate directionally up spatial gradients of cAMP. Cells lacking Mek1 display a similar phenotype. Overexpression of Mek1 in torA- partially restores chemotaxis, whereas overexpression of TorA in mek1- does not rescue the chemotactic phenotype. Regardless of the genetic background, TorA overexpressing cells stop growing when separated from a substrate. Surprisingly, TorA-green fluorescent protein (GFP) is clustered near one end of mitochondria. Deletion analysis of the TorA protein reveals distinct regions for chemotactic function, mitochondrial localization, and the formation of clusters. TorA is associated with a round structure within the mitochondrion that shows enhanced staining with the mitochondrial dye Mitotracker. Cells overexpressing TorA contain many more of these structures than do wild-type cells. These TorA-containing structures resist extraction with Triton X-100, which dissolves the mitochondria. The characterization of TorA demonstrates an unexpected link between mitochondrial function, the chemotactic response, and the capacity to grow in suspension.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.