ArticleThe Journal of cell biology2001
Chromosome movement in mitosis requires microtubule anchorage at spindle poles.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 123 citations in OpenAlex.
- Synaptic vesicle glycoprotein 2 enables viable aneuploidy following centrosome amplification.Genetics · 2025Article
- The post-translational modification of NuMA in cancer cells is a new target for cancer eradication.Cell death & disease · 2025Review
- Force by minus-end motors Dhc1 and Klp2 collapses the S. pombe spindle after laser ablation.Biophysical journal · 2022Article
- WDR62 localizes katanin at spindle poles to ensure synchronous chromosome segregation.The Journal of cell biology · 2021Article
- Mitotic checkpoint regulator RAE1 promotes tumor growth in colorectal cancer.Cancer science · 2021Article
- Mud binds the kinesin-14 Ncd inBiochemistry and biophysics reports · 2021Article
- A non-canonical function for Centromere-associated protein-E controls centrosome integrity and orientation of cell division.Communications biology · 2021Article
- The Modified Phenanthridine PJ34 Unveils an Exclusive Cell-Death Mechanism in Human Cancer Cells.Cancers · 2020Review
- Basal Body Protein TbSAF1 Is Required for Microtubule Quartet Anchorage to the Basal Bodies inmBio · 2020Article
- The phenanthrene derivative PJ34 exclusively eradicates human pancreatic cancer cells in xenografts.Oncotarget · 2019Article
- Maize VKS1 Regulates Mitosis and Cytokinesis During Early Endosperm Development.The Plant cell · 2019Article
- Kinesin-14 motor protein KIFC1 participates in DNA synthesis and chromatin maintenance.Cell death & disease · 2019Article
- p37/UBXN2B regulates spindle orientation by limiting cortical NuMA recruitment via PP1/Repo-Man.The Journal of cell biology · 2018Article
- Isolation and characterization of string-forming female germline stem cells from ovaries of neonatal mice.The Journal of biological chemistry · 2017Article
- Mapping Load-Bearing in the Mammalian Spindle Reveals Local Kinetochore Fiber Anchorage that Provides Mechanical Isolation and Redundancy.Current biology : CB · 2017Article
- Review
- Exclusive destruction of mitotic spindles in human cancer cells.Oncotarget · 2017Article
- Metaphase Spindle Assembly.Biology · 2017Review
- The mitotic kinesin-14 KlpA contains a context-dependent directionality switch.Nature communications · 2017Article
- Mechanical properties of spindle poles are symmetrically balanced.Biophysics and physicobiology · 2017Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Anchorage of microtubule minus ends at spindle poles has been proposed to bear the load of poleward forces exerted by kinetochore-associated motors so that chromosomes move toward the poles rather than the poles toward the chromosomes. To test this hypothesis, we monitored chromosome movement during mitosis after perturbation of nuclear mitotic apparatus protein (NuMA) and the human homologue of the KIN C motor family (HSET), two noncentrosomal proteins involved in spindle pole organization in animal cells. Perturbation of NuMA alone disrupts spindle pole organization and delays anaphase onset, but does not alter the velocity of oscillatory chromosome movement in prometaphase. Perturbation of HSET alone increases the duration of prometaphase, but does not alter the velocity of chromosome movement in prometaphase or anaphase. In contrast, simultaneous perturbation of both HSET and NuMA severely suppresses directed chromosome movement in prometaphase. Chromosomes coalesce near the center of these cells on bi-oriented spindles that lack organized poles. Immunofluorescence and electron microscopy verify microtubule attachment to sister kinetochores, but this attachment fails to generate proper tension across sister kinetochores. These results demonstrate that anchorage of microtubule minus ends at spindle poles mediated by overlapping mechanisms involving both NuMA and HSET is essential for chromosome movement during mitosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.