ArticleJournal of bacteriology2001
Systematic identification of selective essential genes in Helicobacter pylori by genome prioritization and allelic replacement mutagenesis.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
99 citing papers in PubMed, 155 citations in OpenAlex.
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- Helicobacter pylori initiates successful gastric colonization by utilizing L-lactate to promote complement resistance.Nature communications · 2023Article
- The TNF-Alpha Inducing Protein is Associated With Gastric Inflammation and Hyperplasia in a Murine Model ofFrontiers in pharmacology · 2022Article
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- Investigation of LuxS-mediated quorum sensing inJournal of medical microbiology · 2020Article
- High-resolution mapping reveals that microniches in the gastric glands control Helicobacter pylori colonization of the stomach.PLoS biology · 2019Article
- Lipoproteins and Their Trafficking to the Outer Membrane.EcoSal Plus · 2019Review
- Active site remodeling during the catalytic cycle in metal-dependent fructose-1,6-bisphosphate aldolases.The Journal of biological chemistry · 2018Article
- Immunogenicity and efficacy following sequential parenterally-administered doses of Salmonella Enteritidis COPS:FliC glycoconjugates in infant and adult mice.PLoS neglected tropical diseases · 2018Article
- Proteome Data Improves Protein Function Prediction in the Interactome ofMolecular & cellular proteomics : MCP · 2018Article
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- Helicobacter pylori gene silencing in vivo demonstrates urease is essential for chronic infection.PLoS pathogens · 2017Article
- Quantum changes in Helicobacter pylori gene expression accompany host-adaptation.DNA research : an international journal for rapid publication of reports on genes and genomes · 2017Article
- Synthesis and Antimicrobial Evaluation of Amixicile-Based Inhibitors of the Pyruvate-Ferredoxin Oxidoreductases of Anaerobic Bacteria and Epsilonproteobacteria.Antimicrobial agents and chemotherapy · 2016Article
- Comparative Genomics Revealed Multiple Helicobacter pylori Genes Associated with Biofilm Formation In Vitro.PloS one · 2016Article
- Expansion of the tetracycline-dependent regulation toolbox for Helicobacter pylori.Applied and environmental microbiology · 2015Article
- Helicobacter pylori CheZ(HP) and ChePep form a novel chemotaxis-regulatory complex distinct from the core chemotaxis signaling proteins and the flagellar motor.Molecular microbiology · 2015Article
39 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A comparative genomic approach was used to identify Helicobacter pylori 26695 open reading frames (ORFs) which are conserved in H. pylori J99 but highly diverged in other eubacteria. A survey of selected pathways of central intermediary metabolism was also carried out, and genes with a potentially selective role in H. pylori were identified. Forty-five ORFs identified in these two analyses were screened using a rapid vector-free allelic replacement mutagenesis technique, and 33 were shown to be essential in vitro. Notably, 13 ORFs gave essentiality results which are unexpected in view of their known or proposed functions, and phylogenetic analysis was used to investigate the annotation of 7 such ORFs which are highly diverged. We propose that the products of a number of these H. pylori-specific essential genes may be suitable targets for novel anti-H. pylori therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.