ArticleJournal of bacteriology2001
Novel genes affecting urease acivity in Actinobacillus pleuropneumoniae.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Selective inhibition of NikA mediated Ni(II) import in E. coli by the Indium(III)-EDTA complex.Metallomics : integrated biometal science · 2025Article
- Comparative genomics of a vertically transmitted thiotrophic bacterial ectosymbiont and its close free-living relative.Molecular ecology resources · 2024Article
- Genomic insight into strategy, interaction and evolution of nitrifiers in metabolizing key labile-dissolved organic nitrogen in different environmental niches.Frontiers in microbiology · 2023Article
- Rationally designedAnimal diseases · 2021Article
- Comparative Genomics ofInternational journal of genomics · 2020Article
- Ni(II) Interactions in BorealFrontiers in microbiology · 2019Article
- Isolation and molecular characterization of a urease-negative Actinobacillus pleuropneumoniae mutant.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2018Article
- Gene and genome-centric analyses of koala and wombat fecal microbiomes point to metabolic specialization forPeerJ · 2017Article
- Article
- Biosynthesis of the urease metallocenter.The Journal of biological chemistry · 2013Review
- Article
- Investigating the genome diversity of B. cereus and evolutionary aspects of B. anthracis emergence.Genomics · 2011Article
- New plasmid tools for genetic analysis of Actinobacillus pleuropneumoniae and other pasteurellaceae.Applied and environmental microbiology · 2009Article
- Immunoproteomic analysis of outer membrane proteins and extracellular proteins of Actinobacillus pleuropneumoniae JL03 serotype 3.BMC microbiology · 2009Article
- Oligomeric structure and functional characterization of the urea transporter from Actinobacillus pleuropneumoniae.Journal of molecular biology · 2009Article
- Functional characterization of Actinobacillus pleuropneumoniae urea transport protein, ApUT.American journal of physiology. Regulatory, integrative and comparative physiology · 2009Article
- Comparative genomic analyses of nickel, cobalt and vitamin B12 utilization.BMC genomics · 2009Article
- Interplay of metal ions and urease.Metallomics : integrated biometal science · 2009Review
- Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China.PloS one · 2008Article
- Characterization of the urease operon of Brucella abortus and assessment of its role in virulence of the bacterium.Infection and immunity · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Characterization of a series of urease-negative transposon mutations of Actinobacillus pleuropneumoniae revealed that many of the mutants had insertions 2 to 4 kbp upstream of the urease gene cluster. A 5-kbp upstream region of DNA was sequenced and found to contain six open reading frames (ORFs) transcribed in the same orientation as the urease genes. As well, a partial ORF, apuR, 202 bp upstream of these six ORFs, is transcribed in the opposite orientation. The predicted product of this partial ORF shows homology with many members of the LysR family of transcriptional regulators. Five of the ORFs (cbiKLMQO) appear to form an operon encoding a putative nickel and cobalt periplasmic permease system. The cbiM and cbiQ genes encode proteins that have sequence similarity with known cobalt transport membrane proteins, and the cbiO gene encodes a cobalt transport ATP-binding protein homologue. The product of the cbiK gene is predicted to be the periplasmic-binding-protein component of the system, though it does not show any sequence similarity with CbiN, the cobalt-binding periplasmic protein. Escherichia coli clones containing this putative transport operon together with the urease genes of A. pleuropneumoniae were urease positive when grown in unsupplemented Luria-Bertani broth, whereas a clone containing only the minimal urease gene cluster required the addition of high concentrations of NiCl(2) for full urease activity. This result supports the hypothesis that nickel is a substrate for this permease system. The sixth ORF, utp, appears to encode an integral membrane protein which has significant sequence identity with mammalian urea transport proteins, though its function in A. pleuropneumoniae remains to be determined.
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