ArticleGenome research2001
Computer-based methods for the mouse full-length cDNA encyclopedia: real-time sequence clustering for construction of a nonredundant cDNA library.
Article in Genome research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- The juxtaparanodal proteins CNTNAP2 and TAG1 regulate diet-induced obesity.Mammalian genome : official journal of the International Mammalian Genome Society · 2012Article
- Construction and characterization of a goat mammary gland cDNA library.Molecular biotechnology · 2008Article
- Article
- A collection of 10,096 indica rice full-length cDNAs reveals highly expressed sequence divergence between Oryza sativa indica and japonica subspecies.Plant molecular biology · 2007Article
- CAFTAN: a tool for fast mapping, and quality assessment of cDNAs.BMC bioinformatics · 2006Article
- EGassembler: online bioinformatics service for large-scale processing, clustering and assembling ESTs and genomic DNA fragments.Nucleic acids research · 2006Article
- Cytoskeletal rearrangements in synovial fibroblasts as a novel pathophysiological determinant of modeled rheumatoid arthritis.PLoS genetics · 2005Article
- Mistaken identifiers: gene name errors can be introduced inadvertently when using Excel in bioinformatics.BMC bioinformatics · 2004Article
- Comparative genomics of Physcomitrella patens gametophytic transcriptome and Arabidopsis thaliana: implication for land plant evolution.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Impact of alternative initiation, splicing, and termination on the diversity of the mRNA transcripts encoded by the mouse transcriptome.Genome research · 2003Article
- Targeting a complex transcriptome: the construction of the mouse full-length cDNA encyclopedia.Genome research · 2003Article
- Annotation and analysis of 10,000 expressed sequence tags from developing mouse eye and adult retina.Genome biology · 2003Article
- A computer-based method of selecting clones for a full-length cDNA project: simultaneous collection of negligibly redundant and variant cDNAs.Genome research · 2002Article
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Authors and funding
7 authors.
Funding
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Abstract
We developed computer-based methods for constructing a nonredundant mouse full-length cDNA library. Our cDNA library construction process comprises assessment of library quality, sequencing the 3' ends of inserts and clustering, and completing a re-array to generate a nonredundant library from a redundant one. After the cDNA libraries are generated, we sequence the 5' ends of the inserts to check the quality of the library; then we determine the sequencing priority of each library. Selected libraries undergo large-scale sequencing of the 3' ends of the inserts and clustering of the tag sequences. After clustering, the nonredundant library is constructed from the original libraries, which have redundant clones. All libraries, plates, clones, sequences, and clusters are uniquely identified, and all information is saved in the database according to this identifier. At press time, our system has been in place for the past two years; we have clustered 939,725 3' end sequences into 127,385 groups from 227 cDNA libraries/sublibraries (see http://genome.gse.riken.go.jp/).
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