ArticleGenes & development2001
Functionally distinct, sequence-specific replicator and origin elements are required for Drosophila chorion gene amplification.
Article in Genes & development, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 79 citations in OpenAlex.
- Where and when to start: Regulating DNA replication origin activity in eukaryotic genomes.Nucleus (Austin, Tex.) · 2023Review
- Nucleoporins facilitate ORC loading onto chromatin.Cell reports · 2022Article
- Article
- An in vivo gene amplification system for high level expression in Saccharomyces cerevisiae.Nature communications · 2022Article
- Anatomy and evolution of a DNA replication origin.Chromosoma · 2021Article
- MYCN is amplified during S phase, and c‑myb is involved in controlling MYCN expression and amplification in MYCN‑amplified neuroblastoma cell lines.Molecular medicine reports · 2019Article
- Study of the Ability of the gypsy Insulator to Stabilize Amplification of the chorion Replication Origin of Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2018Article
- The Histone Variant H3.3 Is Enriched at Drosophila Amplicon Origins but Does Not Mark Them for Activation.G3 (Bethesda, Md.) · 2016Article
- The hunt for origins of DNA replication in multicellular eukaryotes.F1000prime reports · 2015Review
- The chromatin backdrop of DNA replication: lessons from genetics and genome-scale analyses.Biochimica et biophysica acta · 2012Review
- Comprehensive identification and annotation of cell type-specific and ubiquitous CTCF-binding sites in the human genome.PloS one · 2012Article
- Analysis of a Drosophila amplicon in follicle cells highlights the diversity of metazoan replication origins.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Integrative analysis of gene amplification in Drosophila follicle cells: parameters of origin activation and repression.Genes & development · 2011Article
- Intrinsically bent DNA sites in the Drosophila melanogaster third chromosome amplified domain.Molecular genetics and genomics : MGG · 2009Article
- Chromatin insulators: regulatory mechanisms and epigenetic inheritance.Molecular cell · 2008Review
- Conservation of epigenetic regulation, ORC binding and developmental timing of DNA replication origins in the genus Drosophila.Genetics · 2007Article
- Drosophila follicle cell amplicons as models for metazoan DNA replication: a cyclinE mutant exhibits increased replication fork elongation.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Differential binding of replication proteins across the human c-myc replicator.Molecular and cellular biology · 2006Article
- Coordination of replication and transcription along a Drosophila chromosome.Genes & development · 2004Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To meet the demand for the rapid synthesis of chorion (eggshell) proteins, Drosophila ovarian follicle cells amplify the chromosomal loci containing the chorion gene clusters up to 60-fold. Amplification occurs by repeated firing of one or more origins located within each gene cluster. Deletion analyses of transgenic constructs derived from the third chromosome cluster have identified a 320-bp amplification control element (ACE3) required for amplification, as well as several stimulatory amplification enhancing regions (AERs). Two-dimensional (2D) gel analyses have identified multiple DNA replication initiation sites (origins) that partially overlap in location with ACE3 and the AERs. To further study sequence requirements for amplification, a vector was used in which transgenic constructs are protected from chromosomal position effects by flanking insulator elements, the suppressor Hairy-wing protein binding site (SHWBS). Using the buffered vector, the 320-bp ACE3 and an 884-bp element designated ori-beta were found to be necessary and sufficient for amplification. Two-dimensional gels revealed that ori-beta was acting as the origin. In contrast, origin activity could not be detected for ACE3. An insulator placed between ACE3 and ori-beta inhibited amplification, indicating that ACE3 activates ori-beta in cis. The results suggest that ACE3 acts as a replicator and support and extend the replicator model for the organization of metazoan chromosomal replicons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.