ArticleThe EMBO journal2001
Modulation of Drosophila heat shock transcription factor activity by the molecular chaperone DROJ1.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 85 citations in OpenAlex.
- Variability in mountain pine beetle (Coleoptera: Curculionidae) gene expression in response to increasing concentrations of species-specific double-stranded RNA.Journal of insect science (Online) · 2026Article
- Chronic exposure to the star polycation (SPc) nanocarrier in the larval stage adversely impairs life history traits in Drosophila melanogaster.Journal of nanobiotechnology · 2022Article
- The complex evolution of the metazoan HSP70 gene family.Scientific reports · 2021Article
- Feedback regulation of heat shock factor 1 (Hsf1) activity by Hsp70-mediated trimer unzipping and dissociation from DNA.The EMBO journal · 2020Article
- Heat Shock Factor Is Involved in Regulating the Transcriptional Expression of Two Potential Hsps (Frontiers in physiology · 2020Article
- Variant-specific and reciprocal Hsp40 functions in Hsp104-mediated prion elimination.Molecular microbiology · 2018Article
- Control of RUNX-induced repression of Notch signaling by MLF and its partner DnaJ-1 during Drosophila hematopoiesis.PLoS genetics · 2017Article
- Differential centrifugation-based biochemical fractionation of the Drosophila adult CNS.Nature protocols · 2014Article
- Temperature-dependent resetting of the molecular circadian oscillator in Drosophila.Proceedings. Biological sciences · 2014Article
- Suppression of polyglutamine protein toxicity by co-expression of a heat-shock protein 40 and a heat-shock protein 110.Cell death & disease · 2013Article
- Article
- To fold or not to fold: modulation and consequences of Hsp90 inhibition.Future medicinal chemistry · 2009Review
- Loss of Hsp70 in Drosophila is pleiotropic, with effects on thermotolerance, recovery from heat shock and neurodegeneration.Genetics · 2006Article
- The cellular chaperone heat shock protein 90 facilitates Flock House virus RNA replication in Drosophila cells.Journal of virology · 2005Article
- On mechanisms that control heat shock transcription factor activity in metazoan cells.Cell stress & chaperones · 2004Review
- CHIP activates HSF1 and confers protection against apoptosis and cellular stress.The EMBO journal · 2003Article
- Calmodulin is involved in heat shock signal transduction in wheat.Plant physiology · 2003Article
- Specificity of class II Hsp40 Sis1 in maintenance of yeast prion [RNQ+].Molecular biology of the cell · 2003Article
- Biological functions of the ISWI chromatin remodeling complex NURF.Genes & development · 2002Article
- Mouse heat shock transcription factor 1 deficiency alters cardiac redox homeostasis and increases mitochondrial oxidative damage.The EMBO journal · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat shock transcription factors (HSFs) play important roles in the cellular response to physiological stress signals. To examine the control of HSF activity, we undertook a yeast two-hybrid screen for proteins interacting with Drosophila HSF. DROJ1, the fly counterpart of the human heat shock protein HSP40/HDJ1, was identified as the dominant interacting protein (15 independent isolates from 58 candidates). Overexpression of DROJ1 in Drosophila SL2 cells delays the onset of the heat shock response. Moreover, RNA interference involving transfection of SL2 cells with double-stranded droj1 RNA depletes the endogenous level of DROJ1 protein, leading to constitutive activation of endogenous heat shock genes. The induction level, modest when DROJ1 was depleted alone, reached maximal levels when DROJ1 and HSP70/HSC70, or DROJ1 and HSP90, were depleted concurrently. Chaperone co-depletion was also correlated with strong induction of the DNA binding activity of HSF. Our findings support a model in which synergistic interactions between DROJ1 and the HSP70/HSC70 and HSP90 chaperones modulate HSF activity by feedback repression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.