ArticleThe EMBO journal2001
t-SNARE dephosphorylation promotes SNARE assembly and exocytosis in yeast.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Key processes required for the different stages of fungal carnivory by a nematode-trapping fungus.PLoS biology · 2023Article
- Article
- Identifying Toxicity Mechanisms Associated with Early Lanthanide Exposure through Multidimensional Genome-Wide Screening.ACS omega · 2022Article
- Exocytosis Proteins: Typical and Atypical Mechanisms of Action in Skeletal Muscle.Frontiers in endocrinology · 2022Review
- The Role of Secretory Pathways inJournal of fungi (Basel, Switzerland) · 2020Review
- Blocking dephosphorylation at Serine 120 residue in t-SNARE SNAP-23 leads to massive inhibition in exocytosis from mast cells.Journal of biosciences · 2018Article
- Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23.Nature communications · 2017Article
- The exocyst in Candida albicans polarized secretion and filamentation.Current genetics · 2016Review
- The yeast sphingolipid signaling landscape.Chemistry and physics of lipids · 2014Review
- Functional analysis of phosphorylation on Saccharomyces cerevisiae syntaxin 1 homologues Sso1p and Sso2p.PloS one · 2010Article
- Stress-induced ceramide-activated protein phosphatase can compensate for loss of amphiphysin-like activity in Saccharomyces cerevisiae and functions to reinitiate endocytosis.The Journal of biological chemistry · 2009Article
- Different domains of the UBL-UBA ubiquitin receptor, Ddi1/Vsm1, are involved in its multiple cellular roles.Molecular biology of the cell · 2008Article
- The meiosis-specific Sid2p-related protein Slk1p regulates forespore membrane assembly in fission yeast.Molecular biology of the cell · 2008Article
- Article
- Targeted disruption of the PME-1 gene causes loss of demethylated PP2A and perinatal lethality in mice.PloS one · 2008Article
- Oxidant resistance in a yeast mutant deficient in the Sit4 phosphatase.Current genetics · 2008Article
- Sphingolipids and membrane biology as determined from genetic models.Prostaglandins & other lipid mediators · 2008Review
- Yarrowia lipolytica vesicle-mediated protein transport pathways.BMC evolutionary biology · 2007Article
- Transcriptional regulation by protein kinase A in Cryptococcus neoformans.PLoS pathogens · 2007Article
- The yeast PH domain proteins Slm1 and Slm2 are targets of sphingolipid signaling during the response to heat stress.Molecular and cellular biology · 2007Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of protein phosphorylation in secretion is not well understood. Here we show that yeast lacking the Snc1,2 v-SNAREs, or bearing a temperature-sensitive mutation in the Sso2 t-SNARE, are rescued at restrictive conditions by the addition of ceramide precursors and analogs to the growth medium. Rescue results from dephosphorylation of the Sso t-SNAREs by a ceramide-activated type 2A protein phosphatase (Sit4) involved in cell cycle control. Sso t-SNARE dephosphorylation correlated with its assembly into complexes with the Sec9 t-SNARE, both in vitro and in vivo, and with an increase in protein trafficking and secretion in cells. SNARE complexes isolated under these conditions contained only Sso and Sec9, suggesting that a t-t-SNARE fusion complex is sufficient to confer exocytosis. Mutation of a single PKA site (Ser79 to Ala79) in Sso1 resulted in a decrease in phosphorylation and was sufficient to confer growth to snc cells at restrictive conditions. Thus, modulation of t-SNARE phosphorylation regulates SNARE complex assembly and membrane fusion in vivo.
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