ArticleApplied and environmental microbiology2001
Portable system for microbial sample preparation and oligonucleotide microarray analysis.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 121 citations in OpenAlex.
- Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform.BMC infectious diseases · 2019Article
- Lab-on-a-Film disposable for genotyping multidrug-resistant Mycobacterium tuberculosis from sputum extracts.Lab on a chip · 2019Article
- Microbiological Sensing Technologies: A Review.Bioengineering (Basel, Switzerland) · 2018Review
- Bacterial capture by peptide-mimetic oligoacyllysine surfaces.Applied and environmental microbiology · 2010Article
- Diagnostic microarrays in hematologic oncology: applications of high- and low-density arrays.Molecular diagnosis & therapy · 2009Review
- An adaptable, portable microarray reader for biodetection.Sensors (Basel, Switzerland) · 2009Article
- Species-specific fungal DNA in airborne dust as surrogate for occupational mycotoxin exposure?International journal of molecular sciences · 2008Article
- Automated analytical microarrays: a critical review.Analytical and bioanalytical chemistry · 2008Review
- Discrimination of Bacillus anthracis and closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microarray.Chemico-biological interactions · 2008Article
- Oligonucleotide microchip for subtyping of influenza A virus.Influenza and other respiratory viruses · 2007Article
- Development and applications of microbial ecogenomic indicators for monitoring water quality: report of a workshop assessing the state of the science, research needs and future directions.Environmental monitoring and assessment · 2006Article
- Evaluation of gel-pad oligonucleotide microarray technology by using artificial neural networks.Applied and environmental microbiology · 2005Article
- DNA analysis with multiplex microarray-enhanced PCR.Nucleic acids research · 2005Article
- Automated purification and suspension array detection of 16S rRNA from soil and sediment extracts by using tunable surface microparticles.Applied and environmental microbiology · 2004Article
- The isotope array, a new tool that employs substrate-mediated labeling of rRNA for determination of microbial community structure and function.Applied and environmental microbiology · 2003Article
- Metagenomic profiling: microarray analysis of an environmental genomic library.Applied and environmental microbiology · 2003Article
- Sequence versus structure for the direct detection of 16S rRNA on planar oligonucleotide microarrays.Applied and environmental microbiology · 2003Article
- Optimization of single-base-pair mismatch discrimination in oligonucleotide microarrays.Applied and environmental microbiology · 2003Article
- Direct profiling of environmental microbial populations by thermal dissociation analysis of native rRNAs hybridized to oligonucleotide microarrays.Applied and environmental microbiology · 2003Article
- Fingerprinting of prokaryotic 16S rRNA genes using oligodeoxyribonucleotide microarrays and virtual hybridization.Nucleic acids research · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a three-component system for microbial identification that consists of (i) a universal syringe-operated silica minicolumn for successive DNA and RNA isolation, fractionation, fragmentation, fluorescent labeling, and removal of excess free label and short oligonucleotides; (ii) microarrays of immobilized oligonucleotide probes for 16S rRNA identification; and (iii) a portable battery-powered device for imaging the hybridization of fluorescently labeled RNA fragments with the arrays. The minicolumn combines a guanidine thiocyanate method of nucleic acid isolation with a newly developed hydroxyl radical-based technique for DNA and RNA labeling and fragmentation. DNA and RNA can also be fractionated through differential binding of double- and single-stranded forms of nucleic acids to the silica. The procedure involves sequential washing of the column with different solutions. No vacuum filtration steps, phenol extraction, or centrifugation is required. After hybridization, the overall fluorescence pattern is captured as a digital image or as a Polaroid photo. This three-component system was used to discriminate Escherichia coli, Bacillus subtilis, Bacillus thuringiensis, and human HL60 cells. The procedure is rapid: beginning with whole cells, it takes approximately 25 min to obtain labeled DNA and RNA samples and an additional 25 min to hybridize and acquire the microarray image using a stationary image analysis system or the portable imager.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.