ArticleGenetics2001
Homologous recombinational repair of double-strand breaks in yeast is enhanced by MAT heterozygosity through yKU-dependent and -independent mechanisms.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 79 citations in OpenAlex.
- Automation protocol for high-efficiency and high-quality genomic DNA extraction from Saccharomyces cerevisiae.PloS one · 2023Article
- Review
- Loss of heterozygosity results in rapid but variable genome homogenization across yeast genetic backgrounds.eLife · 2021Article
- Functions of BLM Helicase in Cells: Is It Acting Like a Double-Edged Sword?Frontiers in genetics · 2021Review
- Chromosome drives via CRISPR-Cas9 in yeast.Nature communications · 2020Article
- Variants of the humanMicrobial cell (Graz, Austria) · 2020Article
- An extra copy of the β-glucosidase gene improved the cellobiose fermentation capability of an engineered3 Biotech · 2019Article
- Review
- Multiplexed precision genome editing with trackable genomic barcodes in yeast.Nature biotechnology · 2018Article
- History of genome editing in yeast.Yeast (Chichester, England) · 2018Review
- Rif1 maintains telomeres and mediates DNA repair by encasing DNA ends.Nature structural & molecular biology · 2017Article
- Spindle Checkpoint Factors Bub1 and Bub2 Promote DNA Double-Strand Break Repair by Nonhomologous End Joining.Molecular and cellular biology · 2015Article
- Cancer risk at low doses of ionizing radiation: artificial neural networks inference from atomic bomb survivors.Journal of radiation research · 2014Review
- Development of an assay to measure mutagenic non-homologous end-joining repair activity in mammalian cells.Nucleic acids research · 2013Article
- Mitochondrial genome maintenance: roles for nuclear nonhomologous end-joining proteins in Saccharomyces cerevisiae.Genetics · 2012Article
- Electron microscopy visualization of DNA-protein complexes formed by Ku and DNA ligase IV.DNA repair · 2012Article
- Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology.PloS one · 2012Article
- Efficient mutagenesis of the rhodopsin gene in rod photoreceptor neurons in mice.Nucleic acids research · 2011Article
- High-level transgene expression by homologous recombination-mediated gene transfer.Nucleic acids research · 2011Article
- Yeast Nej1 is a key participant in the initial end binding and final ligation steps of nonhomologous end joining.The Journal of biological chemistry · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
DNA double-strand breaks (DSBs) are repaired by homologous recombination (HR) and nonhomologous end-joining (NHEJ). NHEJ in yeast chromosomes has been observed only when HR is blocked, as in rad52 mutants or in the absence of a homologous repair template. We detected yKu70p-dependent imprecise NHEJ at a frequency of approximately 0.1% in HR-competent Rad+ haploid cells. Interestingly, yku70 mutation increased DSB-induced HR between direct repeats by 1.3-fold in a haploid strain and by 1.5-fold in a MAT homozygous (a/a) diploid, but yku70 had no effect on HR in a MAT heterozygous (a/alpha) diploid. yku70 might increase HR because it eliminates the competing precise NHEJ (religation) pathway and/or because yKu70p interferes directly or indirectly with HR. Despite the yku70-dependent increase in a/a cells, HR remained 2-fold lower than in a/alpha cells. Cell survival was also lower in a/a cells and correlated with the reduction in HR. These results indicate that MAT heterozygosity enhances DSB-induced HR by yKu-dependent and -independent mechanisms, with the latter mechanism promoting cell survival. Surprisingly, yku70 strains survived a DSB slightly better than wild type. We propose that this reflects enhanced HR, not by elimination of precise NHEJ since this pathway produces viable products, but by elimination of yKu-dependent interference of HR.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.