ArticleGenetics2001
Generation of an isogenic collection of yeast actin mutants and identification of three interrelated phenotypes.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Effects of FSGS-associated mutations on the stability and function of myosin-1 in fission yeast.Disease models & mechanisms · 2015Article
- Dissecting BAR domain function in the yeast Amphiphysins Rvs161 and Rvs167 during endocytosis.Molecular biology of the cell · 2010Article
- Syp1 is a conserved endocytic adaptor that contains domains involved in cargo selection and membrane tubulation.The EMBO journal · 2009Article
- Differential requirements for actin during yeast and mammalian endocytosis.Nature cell biology · 2009Article
- Interaction of the endocytic scaffold protein Pan1 with the type I myosins contributes to the late stages of endocytosis.Molecular biology of the cell · 2007Article
- Improper organization of the actin cytoskeleton affects protein synthesis at initiation.Molecular and cellular biology · 2007Article
- Ascospore formation in the yeast Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2005Review
- Alanine scanning of Arp1 delineates a putative binding site for Jnm1/dynamitin and Nip100/p150Glued.Molecular biology of the cell · 2005Article
- End3p-mediated endocytosis is required for spore wall formation in Saccharomyces cerevisiae.Genetics · 2005Article
- Characterization of Schizosaccharomyces pombe mutants defective in vacuolar acidification and protein sorting.Molecular genetics and genomics : MGG · 2004Article
- Induction of salt and osmotic stress tolerance by overexpression of an intracellular vesicle trafficking protein AtRab7 (AtRabG3e).Plant physiology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A large collection of yeast actin mutations has been previously isolated and used in numerous studies of actin cytoskeletal function. However, the various mutations have been in congenic, rather than isogenic, backgrounds, making it difficult to compare the subtle phenotypes that are characteristic of these mutants. We have therefore placed 27 mutations in an isogenic background. We used a subset of these mutants to compare the degree to which different actin alleles are defective in sporulation, endocytosis, and growth on NaCl-containing media. We found that the three phenotypes are highly correlated. The correlations are specific and not merely a reflection of general growth defects, because the phenotypes are not correlated with growth rates under normal conditions. Significantly, those actin mutants exhibiting the most severe phenotypes in all three processes have altered residues that cluster to a small region of the actin crystal structure previously defined as the fimbrin (Sac6p)-binding site. We examined the relationship between endocytosis and growth on salt and found that shifting wild-type or actin mutant cells to high salt reduces the rate of alpha-factor internalization. These results suggest that actin mutants may be unable to grow on salt because of additive endocytic defects (due to mutation and salt).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.