ArticleGenetics2001
Fine structure analysis of the yeast centrin, Cdc31p, identifies residues specific for cell morphology and spindle pole body duplication.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Who cites it
28 citing papers in PubMed, 66 citations in OpenAlex.
- Two Centrins and Their Posttranslational Modification Modulate the Cell Cycle of Giardia lamblia.MicrobiologyOpen · 2025Article
- Structural Basis for the Functional Diversity of Centrins: A Focus on Calcium Sensing Properties and Target Recognition.International journal of molecular sciences · 2021Review
- Ribosomal Protein L40e Fused With a Ubiquitin Moiety Is Essential for the Vegetative Growth, Morphological Homeostasis, Cell Cycle Progression, and Pathogenicity ofFrontiers in microbiology · 2020Article
- Trypanosoma brucei centrin5 is enriched in the flagellum and interacts with other centrins in a calcium-dependent manner.FEBS open bio · 2019Article
- Vps13 and Cdc31/centrin: Puzzling partners in membrane traffic.The Journal of cell biology · 2017Article
- The Vps13p-Cdc31p complex is directly required for TGN late endosome transport and TGN homotypic fusion.The Journal of cell biology · 2017Article
- A genome-wide function of THSC/TREX-2 at active genes prevents transcription-replication collisions.Nucleic acids research · 2014Article
- Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast.Molecular biology of the cell · 2014Article
- The biochemical effect of Ser166 phosphorylation on Euplotes octocarinatus centrin.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2013Article
- The E144 residue of Scherffelia dubia centrin discriminates between the DNA repair protein XPC and the centrosomal protein Sfi1.FEBS open bio · 2013Article
- Mitotic exit and separation of mother and daughter cells.Genetics · 2012Review
- Such small hands: the roles of centrins/caltractins in the centriole and in genome maintenance.Cellular and molecular life sciences : CMLS · 2012Review
- Review
- Role of centrins 2 and 3 in organelle segregation and cytokinesis in Trypanosoma brucei.PloS one · 2012Article
- Analysis of lanthanide-induced conformational change of the C-terminal domain on centrin.Journal of fluorescence · 2012Article
- The two domains of centrin have distinct basal body functions in Tetrahymena.Molecular biology of the cell · 2011Article
- Influence of centriole number on mitotic spindle length and symmetry.Cytoskeleton (Hoboken, N.J.) · 2010Article
- N-terminal regions of Mps1 kinase determine functional bifurcation.The Journal of cell biology · 2010Article
- Dictyostelium discoideum CenB is a bona fide centrin essential for nuclear architecture and centrosome stability.Eukaryotic cell · 2009Article
- Sus1, Cdc31, and the Sac3 CID region form a conserved interaction platform that promotes nuclear pore association and mRNA export.Molecular cell · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Centrin/Cdc31p is a Ca2+-binding protein related to calmodulin found in the MTOC of diverse organisms. In yeast, Cdc31p localizes to the SPB where it interacts with Kar1p and is required for SPB duplication. Recent findings suggest that centrin also functions elsewhere in the cell. To dissect the functions of Cdc31p, we generated cdc31 mutations chosen only for temperature sensitivity, but otherwise unbiased as to phenotype. Three phenotypes of the cdc31 mutants, temperature sensitivity, G2/M arrest, and cell lysis, were not well correlated, indicating that the mutations may differentially affect Cdc31p's interactions with other proteins. Alleles near the C-terminal region exhibited high G2/M arrest and genetic interactions with kar1-Delta17, suggesting that this region modulates an SPB-related function. Alleles causing high lysis and reduced Kic1p kinase activity mapped to the middle of the gene, suggesting disruption of a KIC1-like function and defects in activating Kic1p. A third region conferred temperature sensitivity without affecting cell lysis or G2/M arrest, suggesting that it defines a third function. Mutations in the C-terminal region were also defective for interaction with Kic1p. Mapping the alleles onto a predicted structure of Cdc31p, we have identified surfaces likely to be important for interacting with both Kar1p and Kic1p.
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