ArticleGenome research2001
An efficient method for high-fidelity BAC/PAC retrofitting with a selectable marker for mammalian cell transfection.
Article in Genome research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 43 citations in OpenAlex.
- Species-Specific Relationships between DNA and Chromatin Properties of CpG Islands in Embryonic Stem Cells and Differentiated Cells.Stem cell reports · 2021Article
- Copy-number variation introduced by long transgenes compromises mouse male fertility independently of pachytene checkpoints.Chromosoma · 2020Article
- Identifying DistalRSC advances · 2013Article
- Evidence for a critical role of gene occlusion in cell fate restriction.Cell research · 2012Article
- ZFX controls the self-renewal of human embryonic stem cells.PloS one · 2012Article
- Generating libraries of iTol2-end insertions at BAC ends using loxP and lox511 Tn10 transposons.BMC genomics · 2011Article
- Genome-wide interrogation of Mammalian stem cell fate determinants by nested chromosome deletions.PLoS genetics · 2010Article
- Replacing the wild type loxP site in BACs from the public domain with lox66 using a lox66 transposon.BMC research notes · 2010Article
- Article
- A novel genetic strategy reveals unexpected roles of the Swi-Snf-like chromatin-remodeling BAF complex in thymocyte development.The Journal of experimental medicine · 2008Article
- A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer region in Cre-mediated recombination.BMC genomics · 2006Article
- Decoding the fine-scale structure of a breast cancer genome and transcriptome.Genome research · 2006Article
- Transgenic rescue of the mouse t complex haplolethal locus Thl1.Mammalian genome : official journal of the International Mammalian Genome Society · 2005Article
- Minimal cross-recombination between wild-type and loxP511 sites in vivo facilitates truncating both ends of large DNA inserts in pBACe3.6 and related vectors.Nucleic acids research · 2005Article
- Mutually exclusive recombination of wild-type and mutant loxP sites in vivo facilitates transposon-mediated deletions from both ends of genomic DNA in PACs.Nucleic acids research · 2004Article
- Retrofitting BACs with G418 resistance, luciferase, and oriP and EBNA-1 - new vectors for in vitro and in vivo delivery.BMC biotechnology · 2003Article
- A reliable and efficient method for deleting operational sequences in PACs and BACs.Nucleic acids research · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Large-scale genomic sequencing projects have provided DNA sequence information for many genes, but the biological functions for most of them will only be known through functional studies. Bacterial artificial chromosomes (BACs) and P1-derived artificial chromosomes (PACs) are large genomic clones stably maintained in bacteria and are very important in functional studies through transfection because of their large size and stability. Because most BAC or PAC vectors do not have a mammalian selection marker, transfecting mammalian cells with genes cloned in BACs or PACs requires the insertion into the BAC/PAC of a mammalian selectable marker. However, currently available procedures are not satisfactory in efficiency and fidelity. We describe a very simple and efficient procedure that allows one to retrofit dozens of BACs in a day with no detectable deletions or unwanted recombination. We use a BAC/PAC retrofitting vector that, on transformation into competent BAC or PAC strains, will catalyze the specific insertion of itself into BAC/PAC vectors through in vivo cre/loxP site-specific recombination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.