ArticlePlant physiology2001
Regulation of a wheat actin-depolymerizing factor during cold acclimation.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 115 citations in OpenAlex.
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- Genome-wide identification of the ADF gene family and expression analysis of responses to cold stress in bitter gourd.BMC plant biology · 2026Article
- Molecular dissection of actin depolymerizing factors in wheat and its progenitors: their dynamic expression under elevated temperature stress.Molecular biology reports · 2025Article
- Effects of low temperature stress on photosynthetic characteristics and antioxidant defense system of flue-cured tobacco.Frontiers in plant science · 2025Article
- Genome-wide identification and comprehensive characterization of the ADF gene family inFrontiers in plant science · 2025Article
- Genome-wide identification of actin-depolymerizing factor family genes in melon (Frontiers in plant science · 2024Article
- Transcriptome analysis of brassinolide under low temperature stress in winter wheat.AoB PLANTS · 2023Article
- Research progress on the roles of actin-depolymerizing factor in plant stress responses.Frontiers in plant science · 2023Review
- Genome-wide identification of actin-depolymerizing factor gene family and their expression patterns under various abiotic stresses in soybean (Frontiers in plant science · 2023Article
- Abiotic Stress-InducedFrontiers in plant science · 2021Article
- Genome-Wide Identification and Low Temperature Responsive Pattern of Actin Depolymerizing Factor (ADF) Gene Family in Wheat (Frontiers in plant science · 2021Article
- Arabidopsis ADF5 Acts as a Downstream Target Gene of CBFs in Response to Low-Temperature Stress.Frontiers in cell and developmental biology · 2021Article
- Genome-Wide Identification and Characterization of Actin-Depolymerizing Factor (International journal of molecular sciences · 2020Article
- Understanding the regulation of overwintering diapause molecular mechanisms in Culex pipiens pallens through comparative proteomics.Scientific reports · 2019Article
- An actin-depolymerizing factor from the halophyte smooth cordgrass, Spartina alterniflora (SaADF2), is superior to its rice homolog (OsADF2) in conferring drought and salt tolerance when constitutively overexpressed in rice.Plant biotechnology journal · 2019Article
- An Overview of Signaling Regulons During Cold Stress Tolerance in Plants.Current genomics · 2017Review
- Large scale phosphoprotein profiling to explore Drosophila cold acclimation regulatory mechanisms.Scientific reports · 2017Article
- Bioinformatics identification of new targets for improving low temperature stress tolerance in spring and winter wheat.BMC bioinformatics · 2017Article
- Plant actin depolymerizing factor: actin microfilament disassembly and more.Journal of plant research · 2017Review
- Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots.Proteome science · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously shown that the wheat (Triticum aestivum) TaADF gene expression level is correlated with the plants capacity to tolerate freezing. Sequence analysis revealed that this gene encodes a protein homologous to members of the actin-depolymerizing factor (ADF)/cofilin family. We report here on the characterization of the recombinant TaADF protein. Assays for ADF activity showed that TaADF is capable of sequestering actin, preventing nucleotide exchange, and inducing actin depolymerization. In vitro phosphorylation studies showed that TaADF is a substrate for a wheat 52-kD kinase. The activity of this kinase is modulated by low temperature during the acclimation period. Western-blot analyses revealed that TaADF is expressed only in cold-acclimated Gramineae species and that the accumulation level is much higher in the freezing-tolerant wheat cultivars compared with the less tolerant ones. This accumulation was found to be regulated by a factor(s) encoded by a gene(s) located on chromosome 5A, the chromosome most often found to be associated with cold hardiness. The induction of an active ADF during cold acclimation and the correlation with an increased freezing tolerance suggest that the protein may be required for the cytoskeletal rearrangements that may occur upon low temperature exposure. These remodelings might be important for the enhancement of freezing tolerance.
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