ArticleMolecular and cellular biology2001
Critical role for the histone H4 N terminus in nucleosome remodeling by ISWI.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 122 papers.
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Who cites it
122 citing papers in PubMed, 218 citations in OpenAlex.
- SNF2L suppresses nascent DNA gap formation to promote DNA synthesis.Nucleic acids research · 2024Article
- ISWI catalyzes nucleosome sliding in condensed nucleosome arrays.Nature structural & molecular biology · 2024Article
- Energy-driven genome regulation by ATP-dependent chromatin remodellers.Nature reviews. Molecular cell biology · 2024Review
- ISWI catalyzes nucleosome sliding in condensed nucleosome arrays.bioRxiv : the preprint server for biology · 2023Article
- Nucleosome density shapes kilobase-scale regulation by a mammalian chromatin remodeler.Nature structural & molecular biology · 2023Article
- Drug addiction unveils a repressive methylation ceiling in EZH2-mutant lymphoma.Nature chemical biology · 2023Article
- The role of auxiliary domains in modulating CHD4 activity suggests mechanistic commonality between enzyme families.Nature communications · 2022Article
- Systematic histone H4 replacement in Arabidopsis thaliana reveals a role for H4R17 in regulating flowering time.The Plant cell · 2022Article
- Reb1, Cbf1, and Pho4 Bias Histone Sliding and Deposition Away from Their Binding Sites.Molecular and cellular biology · 2022Article
- Sophisticated Conversations between Chromatin and Chromatin Remodelers, and Dissonances in Cancer.International journal of molecular sciences · 2021Review
- The yeast ISW1b ATP-dependent chromatin remodeler is critical for nucleosome spacing and dinucleosome resolution.Scientific reports · 2021Article
- The ISWI chromatin remodelling factor NURF is not required for mitotic male X chromosome organisation.microPublication biology · 2021Article
- Remodeler Catalyzed Nucleosome Repositioning: Influence of Structure and Stability.International journal of molecular sciences · 2020Review
- Cancer-Associated Gain-of-Function Mutations Activate a SWI/SNF-Family Regulatory Hub.Molecular cell · 2020Article
- The histone H4 basic patch regulates SAGA-mediated H2B deubiquitination and histone acetylation.The Journal of biological chemistry · 2020Article
- A basic motif anchoring ISWI to nucleosome acidic patch regulates nucleosome spacing.Nature chemical biology · 2020Article
- Characterizing Post-Translational Modifications and Their Effects on Protein Conformation Using NMR Spectroscopy.Biochemistry · 2020Review
- Structure of the RSC complex bound to the nucleosome.Science (New York, N.Y.) · 2019Article
- Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome.eLife · 2019Article
- Structures of the ISWI-nucleosome complex reveal a conserved mechanism of chromatin remodeling.Nature structural & molecular biology · 2019Article
62 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ATPase ISWI can be considered the catalytic core of several multiprotein nucleosome remodeling machines. Alone or in the context of nucleosome remodeling factor, the chromatin accessibility complex (CHRAC), or ACF, ISWI catalyzes a number of ATP-dependent transitions of chromatin structure that are currently best explained by its ability to induce nucleosome sliding. In addition, ISWI can function as a nucleosome spacing factor during chromatin assembly, where it will trigger the ordering of newly assembled nucleosomes into regular arrays. Both nucleosome remodeling and nucleosome spacing reactions are mechanistically unexplained. As a step toward defining the interaction of ISWI with its substrate during nucleosome remodeling and chromatin assembly we generated a set of nucleosomes lacking individual histone N termini from recombinant histones. We found the conserved N termini (the N-terminal tails) of histone H4 essential to stimulate ISWI ATPase activity, in contrast to other histone tails. Remarkably, the H4 N terminus, but none of the other tails, was critical for CHRAC-induced nucleosome sliding and for the generation of regularity in nucleosomal arrays by ISWI. Direct nucleosome binding studies did not reflect a dependence on the H4 tail for ISWI-nucleosome interactions. We conclude that the H4 tail is critically required for nucleosome remodeling and spacing at a step subsequent to interaction with the substrate.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.