ArticleMolecular and cellular biology2001
Transcript lifetime is balanced between stabilizing stem-loop structures and degradation-promoting polyadenylation in plant mitochondria.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 89 citations in OpenAlex.
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- Translation in Mitochondrial Ribosomes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Organellar transcripts dominate the cellular mRNA pool across plants of varying ploidy levels.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Exchange on dynamic encounter networks allows plant mitochondria to collect complete sets of mitochondrial DNA products despite their incomplete genomes.Quantitative plant biology · 2022Article
- Nucleo-cytoplasmic interactions affect the 5' terminal transcription of mitochondrial genes between the isonuclear CMS line UG93A and its maintainer line UG93B of kenaf (Hibiscus cannabinus).Development genes and evolution · 2021Article
- Accurate detection of RNA stem-loops in structurome data reveals widespread association with protein binding sites.RNA biology · 2021Article
- Article
- Mitochondrial mRNA Processing in the Chlorophyte AlgaPlants (Basel, Switzerland) · 2021Article
- The plastid and mitochondrial genomes of Eucalyptus grandis.BMC genomics · 2019Article
- Major contribution of transcription initiation to 5'-end formation of mitochondrial steady-state transcripts in maize.RNA biology · 2019Article
- High transcript abundance, RNA editing, and small RNAs in intergenic regions within the massive mitochondrial genome of the angiosperm Silene noctiflora.BMC genomics · 2015Article
- RNA Editing in Chloroplasts of Spirodela polyrhiza, an Aquatic Monocotelydonous Species.PloS one · 2015Article
- Deep sequencing of the tobacco mitochondrial transcriptome reveals expressed ORFs and numerous editing sites outside coding regions.BMC genomics · 2014Article
- Developmentally-specific transcripts from the ccmFN-rps1 locus in wheat mitochondria.Molecular genetics and genomics : MGG · 2008Article
- A Honglian CMS line of rice displays aberrant F0 of F0F1-ATPase.Plant cell reports · 2007Article
- Isolation and characterization of the cytoplasmic male sterility-associated orf456 gene of chili pepper (Capsicum annuum L.).Plant molecular biology · 2007Article
- Mapping of mitochondrial mRNA termini in Arabidopsis thaliana: t-elements contribute to 5' and 3' end formation.Nucleic acids research · 2007Article
- Transcript levels in plant mitochondria show a tight homeostasis during day and night.Molecular genetics and genomics : MGG · 2006Article
- Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing.The Plant cell · 2006Article
- Polyadenylation and degradation of human mitochondrial RNA: the prokaryotic past leaves its mark.Molecular and cellular biology · 2005Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To determine the influence of posttranscriptional modifications on 3' end processing and RNA stability in plant mitochondria, pea atp9 and Oenothera atp1 transcripts were investigated for the presence and function of 3' nonencoded nucleotides. A 3' rapid amplification of cDNA ends approach initiated at oligo(dT)-adapter primers finds the expected poly(A) tails predominantly attached within the second stem or downstream of the double stem-loop structures at sites of previously mapped 3' ends. Functional studies in a pea mitochondrial in vitro processing system reveal a rapid removal of the poly(A) tails up to termini at the stem-loop structure but little if any influence on further degradation of the RNA. In contrast 3' poly(A) tracts at RNAs without such stem-loop structures significantly promote total degradation in vitro. To determine the in vivo identity of 3' nonencoded nucleotides more accurately, pea atp9 transcripts were analyzed by a direct anchor primer ligation-reverse transcriptase PCR approach. This analysis identified maximally 3-nucleotide-long nonencoded extensions most frequently of adenosines combined with cytidines. Processing assays with substrates containing homopolymer stretches of different lengths showed that 10 or more adenosines accelerate RNA processivity, while 3 adenosines have no impact on RNA life span. Thus polyadenylation can generally stimulate the decay of RNAs, but processivity of degradation is almost annihilated by the stabilizing effect of the stem-loop structures. These antagonistic actions thus result in the efficient formation of 3' processed and stable transcripts.
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