ArticleHuman genetics2000
Multilocus genetic analysis of single interphase cells by spectral imaging.
Article in Human genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- Article
- High Performance DNA Probes for Perinatal Detection of Numerical Chromosome Aberrations.Advanced techniques in biology & medicine · 2015Article
- Multi-Color Spectral Transcript Analysis (SPECTRA) for Phenotypic Characterization of Tumor Cells.Biomolecules · 2013Article
- Bioinformatics tools allow targeted selection of chromosome enumeration probes and aneuploidy detection.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
- Bioinformatic Tools Identify Chromosome-Specific DNA Probes and Facilitate Risk Assessment by Detecting Aneusomies in Extra-embryonic Tissues.Current genomics · 2012Article
- Article
- 'Chromosomal Rainbows' Detect Oncogenic Rearrangements of Signaling Molecules in Thyroid Tumors.The open cell signaling journal · 2010Article
- Kinase expression and chromosomal rearrangements in papillary thyroid cancer tissues: investigations at the molecular and microscopic levels.Journal of physiology and pharmacology : an official journal of the Polish Physiological Society · 2009Article
- DNA probe pooling for rapid delineation of chromosomal breakpoints.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- Rapid mapping of chromosomal breakpoints: from blood to BAC in 20 days.Folia histochemica et cytobiologica · 2009Article
- Notch activation is associated with tetraploidy and enhanced chromosomal instability in meningiomas.Neoplasia (New York, N.Y.) · 2008Article
- Chromosome-specific DNA repeat probes.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2006Article
- Aneuploidy involving chromosome 1 in failed-fertilized human oocytes is unrelated to maternal age.Journal of assisted reproduction and genetics · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Numerical chromosome aberrations are detrimental to early embryonic, fetal and perinatal development of mammals. When fetuses carrying a chromosomal imbalance survive to term, an aberrant gene dosage typically leads to stillbirth or causes a severely altered phenotype. Aneuploidy of any of the 24 chromosomes will negatively impact on human development, and a preimplantation and prenatal genetic diagnosis test should thus score as many chromosomes as possible. Since cells available for analysis are likely to be in interphase, we set out to develop a rapid enumeration procedure based on hybridization of chromosome-specific probes and spectral imaging detection. The probe set was chosen to allow the simultaneous enumeration of ten chromosome types and was expected to detect more than 70% of all numerical chromosome aberrations responsible for spontaneous abortions, i.e., human chromosomes 9, 13, 14, 15, 16, 18, 21, 22, X, and Y. Cell fixation protocols were optimized to achieve the desired detection sensitivity and reproducibility. We were able to resolve and identify ten separate chromosomal signals in interphase nuclei from different types of cells, including lymphocytes, uncultured amniocytes, and blastomeres. In summary, this study demonstrates the strength of spectral imaging, allowing us to construct partial spectral imaging karyotypes for individual interphase cells by assessing the number of each of the target chromosome types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.