ArticleProtein science : a publication of the Protein Society2000
Structure of the Ca2+-regulated photoprotein obelin at 1.7 A resolution determined directly from its sulfur substructure.
Article in Protein science : a publication of the Protein Society, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 151 citations in OpenAlex.
- Fast kinetics of photoprotein emitting species.Scientific reports · 2025Article
- The key residue responsible for the red shift of bioluminescence spectra of light-sensitive CaScientific reports · 2025Article
- Exploring the Light-Emitting Agents inJournal of the American Chemical Society · 2024Article
- Facing the phase problem.IUCrJ · 2023Article
- The Role of Tyr-His-Trp Triad and Water Molecule Near the N1-Atom of 2-Hydroperoxycoelenterazine in Bioluminescence of Hydromedusan Photoproteins: Structural and Mutagenesis Study.International journal of molecular sciences · 2023Article
- Crystal structure of semi-synthetic obelin-v after calcium induced bioluminescence implies coelenteramine as the main reaction product.Scientific reports · 2022Article
- Crystal structure of semisynthetic obelin-v.Protein science : a publication of the Protein Society · 2022Article
- Phosphorus and sulfur SAD phasing of the nucleic acid-bound DNA-binding domain of interferon regulatory factor 4.Acta crystallographica. Section F, Structural biology communications · 2021Article
- Unusual shift in the visible absorption spectrum of an active ctenophore photoprotein elucidated by time-dependent density functional theory.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2021Article
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- The hidden treasure in your data: phasing with unexpected weak anomalous scatterers from routine data sets.Acta crystallographica. Section F, Structural biology communications · 2017Article
- In-vacuum long-wavelength macromolecular crystallography.Acta crystallographica. Section D, Structural biology · 2016Article
- Native SAD is maturing.IUCrJ · 2015Review
- Multi-crystal native SAD analysis at 6 keV.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Direct phase selection of initial phases from single-wavelength anomalous dispersion (SAD) for the improvement of electron density and ab initio structure determination.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Anomalous diffraction in crystallographic phase evaluation.Quarterly reviews of biophysics · 2014Review
- Robust structural analysis of native biological macromolecules from multi-crystal anomalous diffraction data.Acta crystallographica. Section D, Biological crystallography · 2013Article
- Structure of the Archaeoglobus fulgidus orphan ORF AF1382 determined by sulfur SAD from a moderately diffracting crystal.Acta crystallographica. Section D, Biological crystallography · 2012Article
- Structures from anomalous diffraction of native biological macromolecules.Science (New York, N.Y.) · 2012Article
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The crystal structure of the photoprotein obelin (22.2 kDa) from Obelia longissima has been determined and refined to 1.7 A resolution. Contrary to the prediction of a peroxide, the noncovalently bound substrate, coelenterazine, has only a single oxygen atom bound at the C2-position. The protein-coelenterazine 2-oxy complex observed in the crystals is photo-active because, in the presence of calcium ion, bioluminescence emission within the crystal is observed. This structure represents only the second de novo protein structure determined using the anomalous scattering signal of the sulfur substructure in the crystal. The method used here is theoretically different from that used for crambin in 1981 (4.72 kDa) and represents a significant advancement in protein crystal structure determination.
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