ArticleProceedings of the National Academy of Sciences of the United States of America2001
An Agrobacterium VirE2 channel for transferred-DNA transport into plant cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 86 citations in OpenAlex.
- Myosin VIII and XI isoforms interact with the Agrobacterium VirE2 protein and facilitate its transport from the plasma membrane to the perinuclear region during plant transformation.Plant communications · 2026Article
- Beyond a few bases: methods for large DNA insertion and gene targeting in plants.The Plant journal : for cell and molecular biology · 2025Review
- Motif-based endomembrane trafficking.Plant physiology · 2021Article
- Pathways of DNA Transfer to Plants fromAnnual review of phytopathology · 2019Article
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- Blocking single-stranded transferred DNA conversion to double-stranded intermediates by overexpression of yeast DNA REPLICATION FACTOR A.Plant physiology · 2015Article
- The Agrobacterium Ti Plasmids.Microbiology spectrum · 2014Review
- Direct visualization of Agrobacterium-delivered VirE2 in recipient cells.The Plant journal : for cell and molecular biology · 2014Article
- The roles of bacterial and host plant factors in Agrobacterium-mediated genetic transformation.The International journal of developmental biology · 2013Review
- Traversing the Cell: Agrobacterium T-DNA's Journey to the Host Genome.Frontiers in plant science · 2012Article
- New insights into an old story: Agrobacterium-induced tumour formation in plants by plant transformation.The EMBO journal · 2010Review
- Overexpression of several Arabidopsis histone genes increases agrobacterium-mediated transformation and transgene expression in plants.The Plant cell · 2009Article
- Agrobacterium tumefaciens and A. rhizogenes use different proteins to transport bacterial DNA into the plant cell nucleus.Microbial biotechnology · 2009Review
- IMPa-4, an Arabidopsis importin alpha isoform, is preferentially involved in agrobacterium-mediated plant transformation.The Plant cell · 2008Article
- Crystal structure of the Agrobacterium virulence complex VirE1-VirE2 reveals a flexible protein that can accommodate different partners.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- VirE2: a unique ssDNA-compacting molecular machine.PLoS biology · 2008Article
- Single-stranded DNA plant pathogens in Eilat.Plant molecular biology · 2006Article
- Analysis of cell type-specific responses mediated by the type IV secretion system of Helicobacter pylori.Infection and immunity · 2005Article
- Agrobacterium tumefaciens-mediated transformation of plants by the pTF-FC2 plasmid is efficient and strictly dependent on the MobA protein.Plant molecular biology · 2004Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Transferred DNA (T-DNA) transfer from Agrobacterium tumefaciens into eukaryotic cells is the only known example of interkingdom DNA transfer. T-DNA is a single-stranded segment of Agrobacterium's tumor-inducing plasmid that enters the plant cell as a complex with the bacterial virulence proteins VirD2 and VirE2. The VirE2 protein is highly induced on contact of A. tumefaciens with a plant host and has been reported to act in late steps of transfer. One of its previously demonstrated functions is binding to the single-stranded (ss) T-DNA and protecting it from degradation. Recent experiments suggest other functions of the protein. A combination of planar lipid bilayer experiments, vesicle swelling assays, and DNA transport experiments demonstrated that VirE2 can insert itself into artificial membranes and form channels. These channels are voltage gated, anion selective, and single-stranded DNA-specific and can facilitate the efficient transport of single-stranded DNA through membranes. These experiments demonstrate a VirE2 function as a transmembrane DNA transporter, which could have applications in gene delivery systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.