Evidence map›Paper›PMID 11149928›Full record

ArticleThe Journal of cell biology2001

The Telomerase/vault-associated protein TEP1 is required for vault RNA stability and its association with the vault particle.

V A Kickhoefer, Y Liu, L B Kong, B E Snow, P L Stewart, L Harrington, L H Rome

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 98 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Review
  7. The small noncoding RNARNA (New York, N.Y.) · 2024
    Article
  8. Article
  9. The small non-coding RNAbioRxiv : the preprint server for biology · 2024
    Article
  10. Article
  11. Human Vault RNAs: Exploring Their Potential Role in Cellular Metabolism.International journal of molecular sciences · 2024
    Review
  12. Review
  13. Review
  14. Article
  15. Article
  16. Review
  17. Vault RNAs: hidden gems in RNA and protein regulation.Cellular and molecular life sciences : CMLS · 2021
    Review
  18. Review
  19. Review
  20. The vault RNA ofThe Journal of biological chemistry · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 2 countries.

V A KickhoeferDepartment of Biological Chemistry, University of California at Los Angeles, School of Medicine, Los Angeles, California 90095, USA. vkick@mednet.ucla.edu
Y Liu
L B Kong
B E Snow
P L Stewart
L Harrington
L H Rome
University of California, Los Angeles · USAmgen (Canada) · CAInstitute for Molecular Medicine · USOntario Institute for Cancer Research · CAUniversity of Toronto · CA

Funding

NOVEL CYTOPLASMIC LARGE RIBONUCLEOPROTEIN PARTICLER01GM038097 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROME, LEONARD H · 1987 to 2001
$337k
NIA NIH HHS AG8422117NIGMS NIH HHS GM38097
6 · The paper itself

Abstract

Vaults and telomerase are ribonucleoprotein (RNP) particles that share a common protein subunit, TEP1. Although its role in either complex has not yet been defined, TEP1 has been shown to interact with the mouse telomerase RNA and with several of the human vault RNAs in a yeast three-hybrid assay. An mTep1(-/-) mouse was previously generated which resulted in no apparent change in telomere length or telomerase activity in six generations of mTep1-deficient mice. Here we show that the levels of the telomerase RNA and its association with the telomerase RNP are also unaffected in mTep1(-/-) mice. Although vaults purified from the livers of mTep1(-/-) mice appear structurally intact by both negative stain and cryoelectron microscopy, three-dimensional reconstruction of the mTep1(-/-) vault revealed less density in the cap than previously observed for the intact rat vault. Furthermore, the absence of TEP1 completely disrupted the stable association of the vault RNA with the purified vault particle and also resulted in a decrease in the levels and stability of the vault RNA. Therefore, we have uncovered a novel role for TEP1 in vivo as an integral vault protein important for the stabilization and recruitment of the vault RNA to the vault particle.

Indexed as

RNA StabilityAnimalsCarrier ProteinsCryoelectron MicroscopyMiceMice, Inbred C57BLMice, KnockoutPhosphate-Binding ProteinsProtein Processing, Post-TranslationalRatsRNA-Binding ProteinsTelomeraseVault Ribonucleoprotein ParticlesCarrier ProteinsPhosphate-Binding ProteinsRNA-Binding ProteinsTelomeraseTep1 protein, mouseTep1 protein, ratVault Ribonucleoprotein Particles

Identifiers

PMID11149928
PMCPMC2193651
OpenAlexW2127837433

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.