ArticleThe Journal of cell biology2001
NXT1 is necessary for the terminal step of Crm1-mediated nuclear export.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 71 citations in OpenAlex.
- Immune Escape-Related Gene NXT1 as a Potential Prognostic and Therapeutic Target in Hepatocellular Carcinoma.The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology · 2025Article
- Karyopherins in the Remodeling of Extracellular Matrix: Implications in Tendon Injury.Journal of orthopaedics and sports medicine · 2023Article
- Specialization of theGenes & development · 2019Article
- NXT1, a Novel Influenza A NP Binding Protein, Promotes the Nuclear Export of NP via a CRM1-Dependent Pathway.Viruses · 2016Article
- RNA Export through the NPC in Eukaryotes.Genes · 2015Review
- Nuclear export of proteins and drug resistance in cancer.Biochemical pharmacology · 2012Review
- The ins and outs of nuclear trafficking: unusual aspects in apicomplexan parasites.DNA and cell biology · 2009Review
- STRADalpha regulates LKB1 localization by blocking access to importin-alpha, and by association with Crm1 and exportin-7.Molecular biology of the cell · 2008Article
- Formation of a Tap/NXF1 homotypic complex is mediated through the amino-terminal domain of Tap and enhances interaction with nucleoporins.Molecular biology of the cell · 2008Article
- Signal sequences for targeting of gene therapy products to subcellular compartments: the role of CRM1 in nucleocytoplasmic shuttling of the protein switch.Pharmaceutical research · 2007Article
- Controlling protein compartmentalization to overcome disease.Pharmaceutical research · 2007Review
- Geldanamycin, an inhibitor of Hsp90, blocks cytoplasmic retention of progesterone receptors and glucocorticoid receptors via their respective ligand binding domains.The AAPS journal · 2006Article
- Mutations in tap uncouple RNA export activity from translocation through the nuclear pore complex.Molecular biology of the cell · 2006Article
- Identification of a novel Rev-interacting cellular protein.BMC cell biology · 2005Article
- NXT2 is required for embryonic heart development in zebrafish.BMC developmental biology · 2005Article
- hRIP, a cellular cofactor for Rev function, promotes release of HIV RNAs from the perinuclear region.Genes & development · 2004Article
- Optical microwell assay of membrane transport kinetics.Biophysical journal · 2003Article
- Nuclear export of mRNA by TAP/NXF1 requires two nucleoporin-binding sites but not p15.Molecular and cellular biology · 2002Article
- Formation of Tap/NXT1 heterodimers activates Tap-dependent nuclear mRNA export by enhancing recruitment to nuclear pore complexes.Molecular and cellular biology · 2002Article
- Transport into and out of the nucleus.Microbiology and molecular biology reviews : MMBR · 2001Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Soluble factors are required to mediate nuclear export of protein and RNA through the nuclear pore complex (NPC). These soluble factors include receptors that bind directly to the transport substrate and regulators that determine the assembly state of receptor-substrate complexes. We recently reported the identification of NXT1, an NTF2-related export factor that stimulates nuclear protein export in permeabilized cells and undergoes nucleocytoplasmic shuttling in vivo (Black, B.E., L. Lévesque, J.M. Holaska, T.C. Wood, and B.M. Paschal. 1999. Mol. Cell. Biol. 19:8616-8624). Here, we describe the molecular characterization of NXT1 in the context of the Crm1-dependent export pathway. We find that NXT1 binds directly to Crm1, and that the interaction is sensitive to the presence of Ran-GTP. Moreover, mutations in NXT1 that reduce binding to Crm1 inhibit the activity of NXT1 in nuclear export assays. We show that recombinant Crm1 and Ran are sufficient to reconstitute nuclear translocation of a Rev reporter protein from the nucleolus to an antibody accessible site on the cytoplasmic side of the NPC. Further progress on the export pathway, including the terminal step of Crm1 and Rev reporter protein release, requires NXT1. We propose that NXT1 engages with the export complex in the nucleoplasm, and that it facilitates delivery of the export complex to a site on the cytoplasmic side of NPC where the receptor and substrate are released into the cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.