ArticleThe Journal of cell biology2001
A novel role of the budding yeast separin Esp1 in anaphase spindle elongation: evidence that proper spindle association of Esp1 is regulated by Pds1.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed, 128 citations in OpenAlex.
- The AMPK kinase Sak1 activates separase and promotes its nuclear localization during mitosis.The Journal of cell biology · 2026Article
- Outer kinetochore proteins form linear elements to regulate vesicle transport.Journal of cell science · 2026Article
- Securin regulates the spatiotemporal dynamics of separase.The Journal of cell biology · 2025Article
- Securin Regulates the Spatiotemporal Dynamics of Separase.bioRxiv : the preprint server for biology · 2023Article
- Structure and Function of the Separase-Securin Complex.Sub-cellular biochemistry · 2021Review
- Accumulation of Securin on Spindle During Female Meiosis I.Frontiers in cell and developmental biology · 2021Article
- PP2AJournal of cell science · 2020Article
- Inhibition of spindle extension through the yeast S phase checkpoint is coupled to replication fork stability and the integrity of centromeric DNA.Molecular biology of the cell · 2019Article
- Characterization of a novel separase-interacting protein and candidate new securin, Eip1p, in the fungal pathogenMolecular biology of the cell · 2019Article
- Cdk1 phosphorylation of Esp1/Separase functions with PP2A and Slk19 to regulate pericentric Cohesin and anaphase onset.PLoS genetics · 2018Article
- Separase-securin complex: a cunning way to control chromosome segregation.Nature structural & molecular biology · 2017Article
- In Favor of Establishment: Regulation of Chromatid Cohesion in Plants.Frontiers in plant science · 2017Review
- Article
- Structural Insights into Separase Architecture and Substrate Recognition through Computational Modelling of Caspase-Like and Death Domains.PLoS computational biology · 2015Article
- Improved Plasmids for Fluorescent Protein Tagging of Microtubules in Saccharomyces cerevisiae.Traffic (Copenhagen, Denmark) · 2015Article
- Suppression of cell division-associated genes by Helicobacter pylori attenuates proliferation of RAW264.7 monocytic macrophage cells.Scientific reports · 2015Article
- A role for the budding yeast separase, Esp1, in Ty1 element retrotransposition.PLoS genetics · 2015Article
- Comparative analysis of chromosome segregation in human, yeasts and trypanosome.Frontiers in biology · 2014Article
- Defective histone supply causes condensin-dependent chromatin alterations, SAC activation and chromosome decatenation impairment.Nucleic acids research · 2014Article
- Cdc20 and securin overexpression predict short-term breast cancer survival.British journal of cancer · 2014Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae, the metaphase-anaphase transition is initiated by the anaphase-promoting complex-dependent degradation of Pds1, whereby Esp1 is activated to promote sister chromatid separation. Although this is a fundamental step in the cell cycle, little is known about the regulation of Esp1 and how loss of cohesion is coordinated with movement of the anaphase spindle. Here, we show that Esp1 has a novel role in promoting anaphase spindle elongation. The localization of Esp1 to the spindle apparatus, analyzed by live cell imaging, is regulated in a manner consistent with a function during anaphase B. The protein accumulates in the nucleus in G2 and is mobilized onto the spindle pole bodies and spindle midzone at anaphase onset, where it persists into midanaphase. Association with Pds1 occurs during S phase and is required for efficient nuclear targeting of Esp1. Spindle association is not fully restored in pds1 mutants expressing an Esp1-nuclear localization sequence fusion protein, suggesting that Pds1 is also required to promote Esp1 spindle binding. In agreement, Pds1 interacts with the spindle at the metaphase-anaphase transition and a fraction remains at the spindle pole bodies and the spindle midzone in anaphase cells. Finally, mutational analysis reveals that the conserved COOH-terminal region of Esp1 is important for spindle interaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.